+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evodiamine, a constituent of Evodiae fructus, induces anti-proliferating effects in tumor cells



Evodiamine, a constituent of Evodiae fructus, induces anti-proliferating effects in tumor cells



Cancer Science 94(1): 92-98



We found that evodiamine, a major alkaloidal component of Evodiae fructus (Goshuyu in Japan), inhibited proliferation of several tumor cell lines, but had less effect on human peripheral blood mononuclear cells (PBMC). We used human cervical cancer cells, HeLa, as a model to elucidate the molecular mechanisms of evodiamine-induced tumor cell death. The results showed that evodiamine induced oligonucleosomal fragmentation of DNA in HeLa cells and increased the activity of caspase-3, but not that of caspase-1, in vitro. Both evodiamine-induced DNA fragmentation and caspase-3 activity were effectively inhibited by a caspase-3 inhibitor, z-DEVD-fmk (z-Asp-Glu-Val-Asp-fmk). In addition, evodiamine increased the expression of the apoptosis inducer Bax, but decreased the expression of the apoptosis suppressor Bcl-2 in mitochondria. Taken together, our data indicated that evodiamine alters the balance of Bcl-2 and Bax gene expression and induces apoptosis through the caspase pathway in HeLa cells.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010625618

Download citation: RISBibTeXText

PMID: 12708481

DOI: 10.1111/j.1349-7006.2003.tb01358.x


Related references

Antitumor mechanism of evodiamine, a constituent from Chinese herb Evodiae fructus, in human multiple-drug resistant breast cancer NCI/ADR-RES cells in vitro and in vivo. Carcinogenesis 26(5): 968-975, 2005

Determination of evodiamine and rutaecarpine in fructus evodiae by HPLC. Progress In Modern Biomedicine: 12, 2536-2537, 2008

Isolation and quantitative analysis of evodiamine from Evodiae Fructus. Korean Journal of Pharmacognosy 32(2): 98-102, 2001

Determination of evodiamine and rutaecarpine in Fructus Evodiae by HPLE. Journal of Shanghai Medical University 26(6): 449-450, 1999

Assay of evodin, evodiamine and rutaecarpine in Fructus Evodiae by QAMS. Zhongguo Zhong Yao Za Zhi 34(21): 2781-2785, 2009

Development and validation of an UPLC-ESI-MS/MS method for determination of dehydroevodiamine, limonin, evodiamine, and rutaecarpine in Evodiae Fructus. Pharmacognosy Magazine 10(39): 374-383, 2014

Chiral high-performance liquid chromatographic separation of evodiamine enantiomers and rutaecarpine, isolated from Evodiae fructus. Journal of Pharmaceutical and Biomedical Analysis 81-82: 151-159, 2014

Studies on Evodiae Fructus I. Qualitative evaluation of various Evodiae Fructus by pharmacognostical and pharmacological methods. Natural Medicines 49(4): 451-454, 1995

A constituent from Evodiae fructus having inhibitory effect on acetylcholinesterase. Seoul University Journal of Pharmaceutical Sciences 20: 1-11, 1995

Anti-diarrheal effect of water extract of Evodiae fructus in mice. Journal of Ethnopharmacology 73(1/2): 39-45, 2000

Anti-Helicobacter pylori activity of quinolone alkaloids from Evodiae fructus. Biological and Pharmaceutical Bulletin 22(10): 1141-1143, 1999

Anti-allergic activities of 70% methanol extract of Evodiae Fructus (Fruits of Evodia rutaecarpa var.bodinieri) and its components. Natural Medicines 52(6): 470-476, 1998

Quinolone alkaloids from evodiae fructus and their inhibitory effects on monoamine oxidase. Archives of Pharmacal Research 30(4): 397-401, 2007