+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Existence of gene 5 indicates close genomic relationship of Turkey coronavirus to infectious bronchitis virus



Existence of gene 5 indicates close genomic relationship of Turkey coronavirus to infectious bronchitis virus



Acta Virologica 46(2): 107-116



A segment of genomic RNA extending from the 3'-end of the membrane (M) protein gene to the 5'-end of the nucleocapsid (N) protein gene of Turkey coronavirus (TCV) was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The primers were derived from the corresponding sequences of Infectious bronchitis virus (IBV). The PCR products were cloned and sequenced and their nucleic acid structure and similarity to the published sequences of IBV were analyzed. Gene 5 containing two overlapping open reading frames (ORFs), 5a and 5b, was localized between M and N genes of TCV. The overall nucleotide sequences of the amplified regions from TCV isolates shared 88.4% to 91.8% similarity to the corresponding region of IBV strains. The consensus transcription-associated sequence of IBV, CTTAACAA, was highly conserved in the TCV genome with regard to nucleotide sequence and location in terms of the initiation codons of the genes 5 and N. The similarities between the predicted amino acid sequences of ORFs 5a and 5b of TCV isolates and the homologous genes of IBV strains were 85.4% to 94.0%. The results indicate the existence of gene 5 in the genome of TCV and a close relatedness of the TCV gene 5 to the IBV gene 5 in location and nucleotide sequence.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 010630365

Download citation: RISBibTeXText

PMID: 12387503


Related references

Nucleocapsid protein gene sequence analysis reveals close genomic relationship between turkey coronavirus and avian infectious bronchitis virus. Acta Virologica 45(1): 31-38, 2001

Sequence analysis of the turkey coronavirus nucleocapsid protein gene and 3' untranslated region identifies the virus as a close relative of infectious bronchitis virus. Virus Research 65(2): 187-193, 1999

Sequence analysis of the turkey enteric coronavirus nucleocapsid and membrane protein genes: a close genomic relationship with bovine coronavirus. Journal of General Virology 72: 1659-1666, 1991

Differential detection of turkey coronavirus, infectious bronchitis virus, and bovine coronavirus by a multiplex polymerase chain reaction. Journal of Virological Methods 131(1): 86-91, 2006

Recombinational histories of avian infectious bronchitis virus and turkey coronavirus. Archives of Virology 156(10): 1823-1829, 2011

Turkey coronavirus is more closely related to avian infectious bronchitis virus than to mammalian coronaviruses: a review. Avian Pathology 29(3): 207-212, 2000

Detection of a coronavirus from turkey poults in Europe genetically related to infectious bronchitis virus of chickens. Avian Pathology 30(4): 355-368, 2001

Sequencing of coronavirus infectious bronchitis genomic rna three open reading frames in the 5' unique region of virus messenger rna d. Journal of General Virology 66(10): 2253-2258, 1985

Sequencing of coronavirus infectious bronchitis virus genomic rna a 195 base open reading frame encoded by messenger rna b. Gene 29(1-2): 87-92, 1984

Genomic relationship of porcine hemagglutinating encephalomyelitis virus to bovine coronavirus and human coronavirus OC43 as studied by the use of bovine coronavirus S gene-specific probes. Archives of virology40(7): 1215-1223, 1995

Comparison of 3'-end encoding regions of turkey coronavirus isolates from Indiana, North Carolina, and Minnesota with chicken infectious bronchitis coronavirus strains. Intervirology 49(4): 230-238, 2006

Gene 5 of the avian coronavirus infectious bronchitis virus is not essential for replication. Journal of Virology 79(13): 8065-8078, 2005

Coronavirus infectious bronchitis virus partial amino terminal sequencing of spike polypeptide s 2 identifies the sequence arginylarginylphenylalanylarginylarginine at the cleavage site of the spike precursor propolypeptide of infectious bronchitis virus strains beaudette and m 41. Virus Research 4(2): 133-144, 1986

Sequence of the membrane protein gene from avian coronavirus infectious bronchitis virus. Virus Research 1(4): 303-314, 1984

The replicase gene of avian coronavirus infectious bronchitis virus is a determinant of pathogenicity. Plos one 4(10): E7384, 2009