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Expressed sequence tags of young floral buds and characterization of a bud-preferential lectin-like cDNA from Pharbitis nil



Expressed sequence tags of young floral buds and characterization of a bud-preferential lectin-like cDNA from Pharbitis nil



Journal of Plant Biology 43(3): 171-178



To identify the genes involved in flower development, we analyzed 207 expressed sequence tags (ESTs) from a young floral bud cDNA library of Pharbitis nil. Of these, 87 clones (42%) showed significant homology to known protein sequences in the NCBI database. Four of these had not been reported previously in the plant kingdom, indicating that 1.9% of the ESTs were newly identified in plants. Functional categorization revealed that the genes involved in metabolic pathways, such as glycolysis and photosynthesis, were most abundant. Reverse-northern and northern analyses showed that one clone, PnFP161, was expressed preferentially in floral buds. DNA sequence analysis indicated that this clone contained 147 bp of 5'-UTR, 264 bp of 3'-UTR, and an open reading frame of 233 amino acids, thereby sharing 33% identity with a lectin from Calystegia sepium. The C-terminal region of PnFP161 had well-conserved residue with that of the lectins. Southern blot analysis demonstrated that PnFP161 exists as a multigene family.

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Accession: 010635334

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