Extracellular proteins secreted in the culture medium of embryogenic and non-embryogenic cell suspensions of sugarcane (Saccharum sp. L.) cv 78-1
Colmenares-Esqueda, M.; Oropeza, M.; De-Garcia, E.
Acta Cientifica Venezolana 49(3): 160-165
Previous results have shown that some proteins secreted in the culture medium are involved with the formation of embryogenic cells and can modify somatic embryos differentiation. Undifferentiated cell suspensions grown in presence of 13 muM 2,4 dichlorophenoxiacetic acid (2,4-D) and obtained from embryogenic and non-embryogenic callus, were used to study these events in sugarcane plants (cv. V78-1). The cell suspensions were subcultured at 4, 7, 14, and 21 days.