EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Flow cytometric assessment of lymphocyte subsets, lymphoid progenitors, and hematopoietic stem cells in allogeneic stem cell grafts



Flow cytometric assessment of lymphocyte subsets, lymphoid progenitors, and hematopoietic stem cells in allogeneic stem cell grafts



Bone Marrow Transplantation 28(11): 1073-1082, December 1



Currently, bone marrow (BM), cord blood (CB), and G-CSF-mobilized peripheral blood progenitor cells (PBPCs) are the most commonly used sources for allogeneic stem cell transplantation (SCT). The aim of this study was to assess the yields and distribution of lymphocyte subsets, lymphocyte progenitors and hematopoietic stem cells (HSC) in each type of allograft by three-color flow cytometry. The yields of CD34+CD38- HSCs did not differ significantly between BM grafts (2.80+-0.74X106) and leukapheresis products (LPs) (1.82+-0.64X106), and were lowest in CB grafts (0.21+-0.05X106). For most lymphocyte subsets yields were lowest in CB grafts and significantly higher in LPs than in BM grafts. BM grafts, however, contained the highest yields of CD34+CD19+CD20- B cell progenitors and CD19+CD20- B cells. The relative frequencies of the naive CD45RA+CD45RO- phenotype among CD4+ and CD8high T cells were highest in CB grafts (Pltoreq0.001), and higher in LPs than in BM grafts (Pltoreq0.02). The latter finding was in accordance with a preferential G-CSF mobilization of naive T cells relative to the total lymphocyte population (Pltoreq0.014). CD3+CD8low and CD3+CD8lowCD4- subsets, which facilitate engraftment in murine transplantation models, demonstrated a tendency towards lower frequencies among T cells in CB grafts and LPs compared to BM grafts. This observation coincided with a significantly reduced mobilization of subsets potentially enriched for facilitating cells as compared to the total lymphocyte population (Pltoreq0.036). The CD34+ compartment of CB grafts contained a significantly higher percentage (12.1%) of CD34+CD7+CD3- T cell progenitors than those of BM grafts (5.1%) and LPs (3.6%). In addition, CB lymphocytes contained the highest fraction of CD3-CD16/56+ NK cells (Pltoreq0.013) and almost no CD3+CD16/56+ NKT cells (P<0.001) compared to adult cell sources. In summary, LPs, CB allografts and BM allografts differ widely with respect to the cellular composition of their lymphocyte compartments, which is partially affected by a varying mobilization efficiency of G-CSF for distinct lymphocyte subsets.

Accession: 010671045

Download citation: RISBibTeXText

PMID: 11781619

DOI: 10.1038/sj.bmt.1703270



Related references

Flowcytometric assessment of lymphocyte subsets, lymphoid progenitors, and hematopoietic stem cells in allografts. Blood 98(11 Part 2): 334b, November 16, 2001

Allogeneic blood stem cell transplantation Delayed effects of rhG-CSF treatment and stem cell apheresis on kinetics of CD34+ cells subsets and lymphoid subsets in 10 normal donors. Blood 86(10 SUPPL 1): 406A, 1995

Flow cytometric enumeration of cytomegalovirus -specific CD8+ T lymphocytes in allogeneic T-lymphocyte depleted hematopoietic stem cell graft recipients. Cytometry Supplement (10): 117, 2000

Flow-cytometric analysis of myeloid and lymphoid cell subsets following cytokine-mediated ex vivo maturation of peripheral blood stem cells. Zeller, W J, Eisenbrand, G, Hellmann, K Contributions to Oncology; Reduction of anticancer drug toxicity: Pharmacologic, biologic, immunologic and gene therapeutic approaches 105-111, 1995

Number of megakaryocytic progenitors and adhesion molecule expression of stem cells predict platelet engraftment after allogeneic hematopoietic stem cell transplantation. CytoTherapy 9(6): 555-561, 2007

CXCR4-related increase of circulating human lymphoid progenitors after allogeneic hematopoietic stem cell transplantation. Plos One 9(3): E91492-E91492, 2015

Comparison of hematopoietic stem cells and T-cell subsets derived from G-primed allogeneic BM, G-mobilized allogeneic PBSC and unprimed allogeneic BM. Blood 92(10 SUPPL 1 PART 1-2): 179B, Nov 15, 1998

Clinical Significance of Monitoring T Lymphocyte Subsets after Allogeneic Hematopoietic Stem Cell Transplantation. Zhongguo Shi Yan Xue Ye Xue Za Zhi 24(1): 167-172, 2016

Recovery of Early and Mature T Lymphocyte Subsets after Allogeneic Hematopoietic Stem Cell Transplantation. Zhongguo Shi Yan Xue Ye Xue Za Zhi 8(2): 133-135, 2003

Flow cytometric findings in platelets of patients following allogeneic hematopoietic stem cell transplantation. Bone Marrow Transplantation 30(6): 381-387, September, 2002

Allogeneic blood stem cell transplantation: peripheralization and yield of donor-derived primitive hematopoietic progenitor cells (CD34+ Thy-1dim) and lymphoid subsets, and possible predictors of engraftment and graft-versus-host disease. Blood 86(7): 2842-2848, 1995

Mesenchymal stem cells in grafts failed to engraft in the bone marrow microenvironment of a leukemia patient post HLA-match and haplo-identical allogeneic hematopoietic stem cell transplantations. Pediatric Hematology and Oncology 31(4): 389-391, 2014

Impact of cytomegalovirus infections on T lymphocyte subsets in children with β-thalassemia major early after allogeneic hematopoietic stem cell transplantation. Nan Fang Yi Ke Da Xue Xue Bao 32(7): 1008-1011, 2013

Reconstitution of lymphocyte subsets after peripheral blood stem cell transplantation: two-color flow cytometric analysis. Bone Marrow Transplantation 13(4): 377-381, 1994

Platelet flow cytometric findings in patients undergoing conditioning therapy for allogeneic hematopoietic stem cell transplantation. Annals of Hematology 81(8): 454-461, 2002