EurekaMag.com logo
+ Translate

Flow cytometry in marine environmental research


, : Flow cytometry in marine environmental research. Periodicum Biologorum 101(2): 151-155, July

Background and purpose: Exposure of marine organisms to environmental contaminants is a global problem. In order to establish effective pollution, control measurement for water conservation, besides emission and contaminant levels, their effect on hereditary material (DNA) must be taken into consideration. Better understanding of responses of the organism to these environmental impacts could be obtained by flow cytometry analysis of cellular DNA content. Materials and methods: The DNA content of hemocytes of the mussel Mytilus galloprovincialis transferred from uncontaminated mariculture area to the area contaminated with industrial waste waters ("mussel watch") was investigated. The measurement of DNA content using flow cytometry is based on the ability of certain fluorescent dyes (DAPI) to bind stoichiometrically to DNA under appropriate staining conditions. By comparing the intensity of each mussel hemocyte to the intensity of each hemocyte containing normal diploid amounts of DNA, the relative quantity of DNA in the hemocytes of interest is determined. Results and conclusions: For the first time it was shown that hemocyte DNA content in the mussels from the vicinity of Rovinj, Istrian coast, Northern Adriatic, Croatia, was influenced by industrial waste waters. We can conclude that G1-arrest and G2-delay appear to represent an active response to DNA damage in mussel hemocytes caused by environmental contamination. Thus, applications of flow cytometry in marine environmental research give us information about health status of organisms as well as about environmental conditions themselves.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 010671267

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Stchepinsky, V., 1948: Sur la geologie de la region de Joinville. Notes the occurrence of Exogyra couloni in beds of Valanginian age.

Moncayo, N., 1980: Espectro visual y unidades de fotorecepcion en mantidos Stagmatoptera biocellata (Mantodea, Insecta). Publicaciones del Museo Ecuatoriano de Ciencias Naturales Serie Revista, 73-87 No. 2

Charbonnier, A.; Perrin, J.; Lahellec, M., 1970: Effect of milk on hepatic clearance in man. Radioactivity in liver was counted after injection by vein of 100 mu Ci colloidal198Au. Uptake of Au by Kupffer cells became significantly greater soon after drinking 160 to 180 ml pasteurized cow's milk than in the same persons fasting and t...

Yamamura K.; Itoh F.; Maehara N., 1981: Physiological responses induced by 555 minute exposure to intermittent noise. Normal humans were exposed to noise for 555 min, while the temporary threshold shift (TTS) and changes in the circulatory system were measured. The exposure noise employed was pink noise of a trapezoidal form. There were 2 time patterns, A(I) with...

Givulescu Razvan; Rueffle Ludwig, 1971: Lower Pliocene (Pannonian) flora of Maramures, Romania, and its relation to the flora of the Miocene-Pliocene boundary in the northern Tethys region, part 1. Geologie: , Pages 168-188. 1971.

Roques, Pierre; Becquart, Pierre; Ayouba, Ahidjo; Chomont, Nicolas; Kazatchkine, Michel D.; Belec, Laurent; Hocini, Hakim, 2003: Phylogenetic analysis showed a compartmentalization of HIV-1 between breast milk and blood of HIV-infected mothers. Infection Genetics & Evolution 2(3): 212, February

Duchacek, V., 1976: Effect of zinc oxide concentration on the course of thiuram-accelerated sulfur vulcanization. Journal of applied polymer science: 20 (1) 71-78

Kara, H.; Nizamloglu, F.; Aktumsek, A.; Dinc, I.; Cengeloglu, Y., 1999: The investigation of some organochlorine pesticide residues in butter. Organochlorine pesticides (OP) were investigated in commercial butter in the Konya region of Turkey. 94% of samples were contaminated with OP, namely DDT complex (DDT, DDD, DDE and isomers) BHC complex (a-BHC, b-BHC and g-BHC (lindane)), aldrin, d...

Tolins J.P.; Palmer R.M.J.; Moncada S.; Raij L., 1990: Endothelium derived relaxing factor edrf modulates renal hemodynamic responses in vivo. Kidney International 37(1): 379

Marinucci, M. T.; Pieramati, C.; Renieri, C.; Petroselli, L., 1989: The Angora rabbit fibre: data on physical characters. Wool samples were taken from the back of 25 [male][male] and 36 [female][female] aged 25-41 months. All rabbits were sampled in Nov., and 20 of the [female][female] were also sampled in Aug. In [male][male], [female][female] sampled in Nov. and [f...