+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Freshly isolated Peyer's patch, but not spleen, dendritic cells produce interleukin 10 and induce the differentiation of T helper type 2 cells



Freshly isolated Peyer's patch, but not spleen, dendritic cells produce interleukin 10 and induce the differentiation of T helper type 2 cells



Journal of Experimental Medicine 190(2): 229-239



Orally administered antigens often generate immune responses that are distinct from those injected systemically. The role of antigen-presenting cells in determining the type of T helper cell response induced at mucosal versus systemic sites is unclear. Here we examine the phenotypic and functional differences between dendritic cells (DCs) freshly isolated from Peyer's patches (PP) and spleen (SP). Surface phenotypic analysis of CD11c(+) DC populations revealed that PP DCs expressed higher levels of major histocompatibility complex class II molecules, but similar levels of costimulatory molecules and adhesion molecules compared with SP DCs. Freshly isolated, flow cytometrically sorted 98-100% pure CD11c(+) DC populations from PP and SP were compared for their ability to stimulate naive T cells. First, PP DCs were found to be much more potent in stimulating allogeneic T cell proliferation compared with SP DCs. Second, by using naive T cells from ovalbumin peptide-specific T cell receptor transgenic mice, these ex vivo DCs derived from PP, but not from SP, were found to prime for the production of interleukin (IL)-4 and IL-10 (Th2 cytokines). In addition, PP DCs were found to prime T cells for the production of much lower levels of interferon (IFN)-gamma (Th1) compared with SP DCs. The presence of neutralizing antibody against IL-10 in the priming culture dramatically enhanced IFN-gamma production by T cells stimulated with PP DCs. Furthermore, stimulation of freshly isolated PP DCs via the CD40 molecule resulted in secretion of high levels of IL-10, whereas the same stimulus induced no IL-10 secretion from SP DCs. These results suggest that DCs residing in different tissues are capable of inducing distinct immune responses and that this may be related to the distinct cytokines produced by the DCs from these tissues.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010683127

Download citation: RISBibTeXText

PMID: 10432286

DOI: 10.1084/jem.190.2.229


Related references

Dendritic cells from Peyer's patch and spleen induce different T helper cell responses. Journal of Interferon and Cytokine Research 18(2): 103-115, 1998

Vicia villosa agglutinin separates freshly isolated Peyer's Patch T cells into interleukin 5- or interleukin 2-producing subsets. Journal of Experimental Medicine 169(4): 1491-1496, 1989

Dendritic Cells from Spleen, Mesenteric Lymph Node and Peyer's Patch Can Induce the Production of Both Il-4 and Ifn- from Primary Cultures of Naive Cd4 + T Cells in a Dose-Dependent Manner. Cytotechnology 43(1-3): 49-55, 2003

FACS-sorted spleen and Peyer's patch dendritic cells induce different responses in Th0 clones. Advances in Experimental Medicine and Biology 417: 357-362, 1997

Dendritic Cells from Spleen, Mesenteric Lymph Node and Peyer's Patch Can Induce the Production of Both IL-4 and IFN-gamma from Primary Cultures of Naive CD4(+) T Cells in a Dose-Dependent Manner. Cytotechnology 43(1-3): 49-55, 2003

Peyer's patch dendritic cells capturing oral antigen interact with antigen-specific T cells and induce gut-homing CD4(+)CD25(+) regulatory T cells in Peyer's patches. Annals of the new York Academy of Sciences 1029: 366-370, 2004

Human dendritic cells induce the differentiation of interleukin-21-producing T follicular helper-like cells through interleukin-12. Immunity 31(1): 158-169, 2009

Distinct T cell priming by freshly isolated dendritic cells from Peyers patch and spleen. FASEB Journal 13(4 Part 1): A606, 1999

Peyer's patch dendritic cells: isolation and functional comparison with murine spleen dendritic cells. Immunobiology 168(3-5): 380-390, 1984

Antigen presentation by Peyer's patch cells can induce both Th1- and Th2-type responses depending on antigen dosage, but a different cytokine response pattern from that of spleen cells. Bioscience Biotechnology and Biochemistry 66(5): 963-969, 2002

Murine Peyer's patch dendritic cells prime naïve CD4(+) T cells to produce interferon-gamma. Cytotechnology 40(1-3): 31-38, 2002

CD11b+ Peyer's patch dendritic cells secrete IL-6 and induce IgA secretion from naive B cells. Journal of Immunology 171(7): 3684-3690, 2003

Induction of IgA B cell differentiation of bone marrow-derived B cells by Peyer's patch autoreactive helper T cells. Immunological Investigations 24(5): 701-711, 1995

Cutting Edge: Peyer's Patch Plasmacytoid Dendritic Cells (pDcs) Produce Low Levels of Type I Interferons: Possible Role for Il-10, Tgf, and Prostaglandin E2 in Conditioning a Unique Mucosal pDc Phenotype. The Journal of Immunology 179(5): 2690-2694, 2007

CD8+ immunoregulatory cells in the graft-versus-host reaction: CD8 T cells activate dendritic cells to secrete interleukin-12/interleukin-18 and induce T helper 1 autoantibody. Immunology 109(4): 476-486, 2003