EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Functional differences between cardiac and renal isoforms of the rat Na+-Ca2+ exchanger NCX1 expressed in Xenopus oocytes


Functional differences between cardiac and renal isoforms of the rat Na+-Ca2+ exchanger NCX1 expressed in Xenopus oocytes



Journal of Physiology 529 Pt 3: 599-610



ISSN/ISBN: 0022-3751

PMID: 11118492

DOI: 10.1111/j.1469-7793.2000.00599.x

1. The transcript of the Na+-Ca2+ exchanger gene NCX1 undergoes alternative splicing to produce tissue-specific isoforms. The cloned NCX1 isoforms were expressed in Xenopus oocytes and studied using a two-electrode voltage clamp method to measure Na+-Ca2+ exchanger activity. 2. The cardiac isoform (NCX1.1) expressed in oocytes was less sensitive to depolarizing voltages and to activation by (Ca2+)i than the renal isoform (NCX1.3). 3. The cardiac isoform of NCX1 is more sensitive to activation by protein kinase A (PKA) than the renal isoform which may be explained by preferential phosphorylation. The cardiac isoform of NCX1 is phosphorylated to a greater extent than the renal isoform. 4. The action of PKA phosphorylation which increases the activity of the cardiac isoform of the Na+-Ca2+ exchanger in oocytes was confirmed in adult rat ventricular cardiomyocytes by measuring Na+-dependent Ca2+ flux. 5. We conclude that alternative splicing of NCX1 confers distinct functional characteristics to tissue-specific isoforms of the Na+-Ca2+ exchanger.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010687609

Download citation: RISBibTeXText

Related references

Functional differences between a subunit isoforms of the rat Na,K-ATPase expressed in Xenopus oocytes. The Journal of Physiology 539(3): 9-80, 2002

Functional differences between alpha subunit isoforms of the rat Na,K-ATPase expressed in Xenopus oocytes. Journal of Physiology 539(Pt 3): 669-680, 2002

Functional characterization and regulation by pH of murine AE2 anion exchanger expressed in Xenopus oocytes. American Journal of Physiology 267(5 Pt 1): C1295-C1307, 1994

Cloning of the mouse cardiac Na(+)-Ca2+ exchanger and functional expression in Xenopus oocytes. Annals of the new York Academy of Sciences 779: 126-128, 1996

The functional unit of the rat renal type IIA Na+/PI cotransporter expressed in Xenopus oocytes is a monomer. Nephrology Dialysis Transplantation 15(9): A29, 2000

Functional characterization of the cloned renal peptide transporter rhaPT expressed in Xenopus laevis oocytes. Pfluegers Archiv European Journal of Physiology 430(4 Suppl): R72, 1995

Functional association of the b1 subunit with human cardiac (hH1) and rat skeletal muscle (m1) sodium channel a subunits expressed in Xenopus oocytes. The Journal of General Physiology 106: 71-91, 1995

Functional association of the beta 1 subunit with human cardiac (hH1) and rat skeletal muscle (mu 1) sodium channel alpha subunits expressed in Xenopus oocytes. Journal of General Physiology 106(6): 1171-1191, 1995

Murine AE2 anion exchanger expressed in xenopus oocytes is regulated by intracellular pH in the physiological range. Journal of the American Society of Nephrology 4(3): 841, 1993

Differential targeting of glucose transporter isoforms heterologously expressed in Xenopus oocytes. Biochemical Journal 290: 707-715, 1993

Acute regulation of the SLC26A3 congenital chloride diarrhoea anion exchanger (DRA) expressed in Xenopus oocytes. Journal of Physiology 549(Pt 1): 3-19, 2003

Transport of proline and hydroxyproline by the neutral amino acid exchanger ASCT1 expressed in Xenopus laevis oocytes. FASEB Journal 17(4-5): Abstract 583, 2003

MRNA-induced expression of the cardiac Na+-Ca2+ exchanger in Xenopus oocytes. Journal of Biological Chemistry 263(29): 14614-14616, 1988

Critical residues of the regulatory pH modifier site in the N-terminal cytoplasmic domain of the AE2 anion exchanger expressed in Xenopus oocytes. Journal of the American Society of Nephrology 11(Program and Abstract Issue): 10A, 2000

Expression of the chick cardiac sodium calcium exchanger in xenopus oocytes. Experientia 45(ABSTR): A46, 1989