+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Fungal endophytes from stalks of tropical maize and grasses: Isolation, identification, and screening for antagonism against Fusarium verticillioides in maize stalks



Fungal endophytes from stalks of tropical maize and grasses: Isolation, identification, and screening for antagonism against Fusarium verticillioides in maize stalks



Biocontrol Science & Technology 9(4): 545-553



A bioassay to screen fungal isolates for endophytic growth and antagonism against Fusarium verticillioides in maize was developed. The method was based on the commonly used toothpick inoculation method followed by measurement of stalk necrosis, and was designed to assure a direct introduction of the endophyte into the plant. Thirty-four fungal endophytes isolated from surface sterilized grass and maize stalks from Costa Rica, and four soil isolates, were tested for antagonism and endophytic growth. Six isolates gave less necrosis (P < 0.05) than the control treated with F. verticillioides alone, but only one isolate, Trichoderma koningii S8, reduced the stalk necrosis when the test was repeated. Reisolations from the stalk showed that none of the isolates were able to completely eliminate F. verticillioides from the maize stalk. It is concluded that F. verticillioides is a very strong competitor that is highly adapted to live in association with maize, and that an effective antagonist against F. verticillioides still remains to be found. The screening assay developed in this study may prove to be a useful tool to study the in vivo interactions between plant pathogens and antagonistic endophytes.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010690727

Download citation: RISBibTeXText

DOI: 10.1080/09583159929505


Related references

Combined Metabarcoding and Co-occurrence Network Analysis to Profile the Bacterial, Fungal and Fusarium Communities and Their Interactions in Maize Stalks. Frontiers in Microbiology 10: 261, 2019

Effect of Ca(OH)2 and NaOH treatments on the nutritive value of maize stalks, sorghum stalks and dry sweet potato vines. J. Animal Prod. U.A.R, 1966: 4, 55-65, 1964

Analysis of microbial taxonomical groups present in maize stalks suppressive to colonization by toxigenic Fusarium spp.: A strategy for the identification of potential antagonists. Biological Control 83: 20-28, 2015

Comparison of chemical contents and in vitro nutrient digestibility of maize stalks from high oil maize with conventional or specific fodder maize. Journal of Animal and Feed Sciences 16: 37-42, 2007

Lodging of maize stalks. II. Effect of some Fusarium species on yield. Analele Institutului de Cercetari pentru Protectia Plantelor 18: 55-71, 1985

Identification of a cis-acting factor modulating the transcription of FUM1, a key fumonisin-biosynthetic gene in the fungal maize pathogen Fusarium verticillioides. Fungal Genetics and Biology 51: 42-49, 2013

Comparison of methods for inoculation of ears and stalks of maize with fusarium moniliforme. Plant Disease 74(12): 952-956, 1990

Studies on methods of testing maize for resistance to Fusarium rot of stalks and ears. Probleme de Protectia Plantelor 9(4): 341-348, 1981

Biodiversity of Fusarium species in ears and stalks of maize plants in Belgium. European Journal of Plant Pathology 131(1): 59-66, 2011

Isolation of sordaria fimicola from maize stalks. Journal of Phytopathology (Berlin) 120(4): 369-371, 1987

Genetic diversity of Fusarium section Liseola (Gibberella fujikuroi) in individual maize stalks. Phytopathology 84(6): 603-607, 1994

Interactions between Fusarium verticillioides and Fusarium graminearum in maize ears and consequences for fungal development and mycotoxin accumulation. Plant Pathology 61(1): 140-151, 2012

Isolation and identification of endophytic bacterial strains from sugarcane stalks and their in vitro antagonism against the red rot pathogen. Sugar Tech 5(1/2): 25-29, 2003

Influence of maize growth stage on fungal movement viability and rot induction in stalks inoculated with colletotrichum graminicola. Phytopathology 77(1): 115, 1987

Comparative study of the nutritive value of maize silage and dried ground maize stalks. Revista da Sociedade Brasileira de Zootecnia(1): 124-132, 1979