Section 11
Chapter 10,714

Genotyping of European isolates of methicillin-resistant Staphylococcus aureus by fluorescent amplified-fragment length polymorphism analysis (FAFLP) and pulsed-field gel electrophoresis (PFGE) typing

Grady, R.; Blanc, D.; Hauser, P.; Stanley, J.

Journal of Medical Microbiology 50(7): 588-593


ISSN/ISBN: 0022-2615
PMID: 11444768
Accession: 010713841

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A representative panel of 50 European MRSA isolates was subjected to genotype analysis by fluorescent amplified-fragment length polymorphism (FAFLP) and by macrorestriction pulsed-field gel electrophoresis (PFGE). Each isolate had a unique profile with FAFLP. To model genetic relationships within the continuing MRSA epidemic, cluster analysis of FAFLP data was made, revealing nine clone complexes of MRSA. Most of these were also found by PFGE. A number of isolates had FAFLP profiles significantly different from others, and might represent emerging epidemic strains.

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