+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

HPLC method for quantification of arginine containing vasopressin



HPLC method for quantification of arginine containing vasopressin



Journal of Liquid Chromatography & Related Technologies 22(4): 551-560



An isocratic technique was developed for the analysis of (Arg8)- vasopressin (AVP) by reverse-phase high performance liquid chromatography (RP-HPLC) using a 220 nm UV detection, Brownlee Spheri-5 ODS column (250 X 4.6 mm, 5 mum, 100 ANG), mobile phase (methanol: 0. 1% aqueous trifluoroacetic acid: : 3:7), and 1.5 mL/min flow rate. The coefficients of variation (C.V.) for precision and proportionality assays were lower than 2% for all concentrations studied. The detection limit, recovery rate, and tailing factor for AVP were 50 ng/mL (signal-to-noise ratio of 3:1), 0.97 +- 0.03, and 0.99- 1. 1, respectively.

(PDF emailed within 0-6 h: $19.90)

Accession: 010732445

Download citation: RISBibTeXText

DOI: 10.1081/JLC-100101680


Related references

Synthesis and some pharmacological properties of deamino 4 threonine 8 d arginine vasopressin and deamino 8 d arginine vasopressin highly potent and specific anti diuretic peptides and 8 d arginine vasopressin and deamino arginine vasopressin. Journal of Medicinal Chemistry 19(6): 842-845, 1976

Development of Isocratic RP-HPLC Method for Separation and Quantification of L-Citrulline and L-Arginine in Watermelons. International Journal of Analytical Chemistry 2018: 4798530-4798530, 2018

Diabetes insipidus patient refractory to vasopressin and 8 d arginine deamino vasopressin absence of antibodies to 8 l arginine vasopressin and 8 d arginine deamino vasopressin and inactivating factor in blood serum. International Journal of Clinical Pharmacology & Biopharmacy 17(2): 84-86, 1979

Alanine glycine 8 arginine vasopressin and valine aspartic acid 8 arginine vasopressin bovine storage forms of arginine vasopressin with natriuretic activity. Science (washington D C)433): 893-896, 1980

1 l 2 hydroxy 3 mercapto propanoic acid analogs of arginine vasopressin 8 d arginine vasopressin and 4 valine 8 d arginine vasopressin. Journal of Medicinal Chemistry 20(9): 1173-1176, 1977

Development and validation of an HPLC-MS/MS method for the determination of arginine-vasopressin receptor blocker conivaptan in human plasma and rat liver microsomes: application to a metabolic stability study. Chemistry Central Journal 12(1): 47-47, 2018

1-(L-2-hydroxy-3-mercaptopropanoic acid)] analogues of arginine-vasopressin, [8-D-arginine]vasopressin, and [4-valine,8-D-arginine]vasopressin. Journal of Medicinal Chemistry 20(9): 1173-1176, 1977

Method development and validation of HPLC tandem/mass spectrometry for quantification of perindopril arginine and amlodipine besylate combination in bulk and pharmaceutical formulations. Research in Pharmaceutical Sciences 12(4): 307-314, 2017

The release of plasminogen activator and factor VIII to lysine vasopressin, arginine vasopressin, I-desamino-8-d-arginine vasopressin, angiotensin and oxytocin in man. British Journal of Haematology 27(2): 363-364, 1974

The release of plasminogen activator and factor 103 to lysine vasopressin arginine vasopressin i deamino 8 d arginine vasopressin angiotensin and oxytocin in man. British Journal of Haematology 27(2): 363-364, 1974

The effect of arginine vasopressin, lysine vasopressin or oxytocin on ADP or arginine vasopressin-induced Ca2+ increase in human platelets. Clinical Physiology (Oxford) 19(4): 305-310, July, 1999

The effects of two analogues of arginine-vasopressin (ornithine-vasopressin and desamino-D-arginine-vasopressin) on kidney function in sheep. Quarterly Journal of Experimental Physiology and Cognate Medical Sciences 63(2): 179-188, 1978

The effects of 2 analogs of arginine vasopressin ornithine vasopressin and deamino d arginine vasopressin on kidney function in sheep. Quarterly Journal of Experimental Physiology and Cognate Medical Sciences 63(2): 179-188, 1978

Use of 3 specific radioimmunoassays in measuring neurohypophyseal hormone content and plasma concentrations of vasopressin oxytocin and 1 desamino 8 d arginine vasopressin in rats after prolonged infusion of 1 desamino 8 d arginine vasopressin. Experientia (Basel) 41(7): 933-935, 1985

Effects of vasopressin analogs 1 deamino 8 d arginine vasopressin 1 deamino 4 valine 8 d arginine vasopressin in the form of sublingual tablets in central diabetes insipidus. International Journal of Clinical Pharmacology Therapy & Toxicology 19(2): 63-68, 1981