+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Headspace solid-phase microextraction analysis of 3-alkyl-2-methoxypyrazines in wines

Headspace solid-phase microextraction analysis of 3-alkyl-2-methoxypyrazines in wines

Journal of Chromatography A 953(1-2): 1-6, 12 April

A procedure to determine 3-alkyl-2-methoxypyrazines in wines is described. It is based on the headspace solid-phase microextraction (HS-SPME) technique after a clean-up of the sample by distillation (previously acidified to pH 0.5) to remove ethanol and other volatile compounds that can interfere in the SPME. Determination is performed by means of capillary gas chromatography using a nitrogen-phosphorus detector. The method allows quantification of 3-isobutyl-2-methoxypyrazine, 3-sec-butyl-2-methoxypyrazine and 3-isopropyl-2-methoxypyrazine at their natural concentration levels and below their sensory thresholds in Cabernet Sauvignon and Merlot wines. The method was successfully applied to experimental red wines and the evolution of their pyrazine contents during the winemaking process was monitored. Pyrazine content increased during the first maceration day but did not change significantly during alcoholic and malolactic fermentation. Final contents in wines were 12-27 ng/l of 3-isobutyl-2-methoxypyrazine and 5-10 ng/l of 3-sec-butyl-2-methoxypyrazine.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 010736446

Download citation: RISBibTeXText

PMID: 12058923

DOI: 10.1016/s0021-9673(02)00123-1

Related references

Implementation of headspace solid-phase-microextraction-GC-MS/MS methodology for determination of 3-alkyl-2-methoxypyrazines in wine. European Food Research and Technology 227(2): 449-461, 2008

Determination of 3-alkyl-2-methoxypyrazines in lady beetle-infested wine by solid-phase microextraction headspace sampling. Journal of Agricultural and Food Chemistry 56(3): 1065-1071, 2008

Headspace solid-phase microextraction method for determining 3-alkyl-2-methoxypyrazines in musts by means of polydimethylsiloxane-divinylbenzene fibres. Journal of Chromatography A 880(1-2): 93-99, 2 June, 2000

Measurement of 3-alkyl-2-methoxypyrazine by headspace solid-phase microextraction in spiked model wines. American Journal of Enology and Viticulture 53(4): 285-288, 2002

Analysis of methoxypyrazines in wine using headspace solid phase microextraction with isotope dilution and comprehensive two-dimensional gas chromatography. Journal of Separation Science 28(9/10): 1075-1082, 2005

Accurate determination of 3-alkyl-2-methoxypyrazines in wines by gas chromatography quadrupole time-of-flight tandem mass spectrometry following solid-phase extraction and dispersive liquid-liquid microextraction. Journal of Chromatography. a 1515: 30-36, 2017

Multidimensional gas chromatography-mass spectrometry determination of 3-alkyl-2-methoxypyrazines in wine and must. A comparison of solid-phase extraction and headspace solid-phase extraction methods. Journal of Chromatography. a 1216(18): 4040-4045, 2009

Assessment of the matrix effect on the headspace solid-phase microextraction (HS-SPME) analysis of chlorophenols in wines. Journal of Separation Science 30(5): 722-730, 2007

Analysis of benzothiazole in Italian wines using headspace solid-phase microextraction and gas chromatography-mass spectrometry. Journal of Agricultural and Food Chemistry 48(4): 1239-1242, 2000

Development of headspace solid-phase microextraction-gas chromatography-mass spectrometry methodology for analysis of terpenoids in Madeira wines. Analytica Chimica Acta 555(2): 191-200, 2006

Headspace Solid Phase Microextraction (HS-SPME), Headspace Solid Phase Dynamic Extraction (HS-SPDE) and Headspace Sorptive Extraction (HSSE) - comparing the methods applied to the analysis of volatile components in herbs. Zeitschrift fur Arznei and Gewurzpflanzen 12(3): 147-153, 2007

Comparison of fully-automated headspace single drop microextraction and headspace solid phase microextraction techniques for rapid analysis of No. 6 solvent residues in edible oil. Microchemical Journal 117: 187-193, 2014

Preparation of novel solid-phase microextraction fibers by sol-gel technology for headspace solid-phase microextraction-gas chromatographic analysis of aroma compounds in beer. Journal of Chromatography, A 1065(2): 287-299, 2005

Headspace solid phase microextraction (SPME) analysis of flavor compounds in wines. Effect of the matrix volatile composition in the relative response factors in a wine model. Journal of Agricultural and Food Chemistry 49(11): 5142-5151, 2001

Volatile compounds of red and white wines by headspace--solid-phase microextraction using different fibers. Journal of Chromatographic Science 42(6): 310-316, 2004