+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Heterogeneous Sp1 mRNAs in human HepG2 cells include a product of homotypic trans-splicing

Heterogeneous Sp1 mRNAs in human HepG2 cells include a product of homotypic trans-splicing

Journal of Biological Chemistry 275(48): 38067-38072

Sp1 is one of the well documented transcription factors, but the whole structure of human Sp1 has not been determined yet. In the present study, we isolated several cDNAs representing two forms of human Sp1 mRNA with different 5'-terminal structures in HepG2 cells. Isolation of a genomic clone established that one of the cDNAs represents the mRNA having consecutive alignment of exons, which allowed deducing the complete amino acid sequence for human Sp1. Another cDNA clone had a surprising structure that possessed an alignment of exons 3-2-3. Both reverse transcriptase-polymerase chain reaction and RNase protection assays confirmed accumulation of the two forms of Sp1 mRNA in HepG2 cells. Because Southern blot analysis suggested that exon 3 is of a single copy in the genome, the cDNA clone having the duplicated sequences for exon 3 appeared to reflect the trans-splicing between pre-mRNAs of human Sp1.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 010747015

Download citation: RISBibTeXText

PMID: 10973950

DOI: 10.1074/jbc.M002010200

Related references

Trans splicing in Oenothera mitochondria: nad1 mRNAs are edited in exon and trans-splicing group II intron sequences. Cell Cambridge 65(3): 473-482, 1991

RNA trans-splicing in flatworms. Analysis of trans-spliced mRNAs and genes in the human parasite, Schistosoma mansoni. Journal of Biological Chemistry 270(37): 21813-9, 1995

Cis- and trans-splicing of mRNAs mediated by tRNA sequences in eukaryotic cells. Proceedings of the National Academy of Sciences of the United States of America 105(19): 6864-6869, 2008

Improvement in RNA trans-splicing signal amplification in cells and animal imaging using smaller pre trans-splicing molecules. Journal of Nuclear Medicine 44(5 Supplement): 373P, 2003

Trans splicing of nuclear pre-mRNAs. Cell 61(7): 1157-1160, 1990

Processing of carnitine octanoyltransferase pre-mRNAs by cis and trans-splicing. Advances in Experimental Medicine and Biology 466: 95-102, 2000

Effect of interleukin-11 on the levels of mRNAs encoding heme oxygenase and haptoglobin in human HepG2 hepatoma cells. Biochemical and Biophysical Research Communications 193(1): 297-302, 1993

Preprotachykinin gene expression in the human basal ganglia: characterization of mRNAs and pre-mRNAs produced by alternate RNA splicing. Brain Research. Molecular Brain Research 12(1-3): 225-231, 1992

Evidence for in vivo Trans splicing of pre-mRNAs in tobacco chloroplasts. Cell, USA 48(1): 111-119, 1987

Editing of pre-mRNAs can occur before cis- and trans-splicing in Petunia mitochondria. Molecular and Cellular Biology 11(8): 4274-4277, 1991