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Hodgkin's disease: immunoglobulin heavy and light chain gene rearrangements revealed in single Hodgkin/Reed-Sternberg cells

Hodgkin's disease: immunoglobulin heavy and light chain gene rearrangements revealed in single Hodgkin/Reed-Sternberg cells

Molecular Pathology 52(1): 37-41

Aim. To corroborate and investigate the nature of Hodgkin/Reed-Sternberg cells (H/R-S) of various subtypes of Hodgkin's disease. Method. Single H/R-S cells were micropicked from frozen sections of tissues affected by Hodgkin's disease. The DNA from these cells was amplified by the polymerase chain reaction (PCR) with immunoglobulin heavy chain (IgH) gene FRIIIa/JH primers and light chain gene family specific primers. Results. Fifty two of 135 isolated cells gave specific reaction products (36%). IgH and Vkappa4 gene rearrangements were found repeatedly in many H/R-S cells from one case of lymphocyte predominant Hodgkin's disease. Repeated Vkappa4 and individual IgH/Vkappa4,2 rearrangements were seen in one case, and individual IgH and Vlambda3/Vkappa4 rearrangements were seen in another case of nodular sclerosis-type Hodgkin's disease. Repeated IgH/Vlambda3 and individual Vlambda2,4 rearrangements, repeated Vkappa4 and individual IgH/Vkappa3 rearrangements, and repeated IgH and individual Vkappa3/Vkappa4 rearrangement were detected, respectively, in three cases of mixed cellularity-type Hodgkin's disease. Repeated and individual IgH rearrangements were found in another two cases of mixed cellularity-type Hodgkin's disease. Conclusion. The H/R-S cells isolated from lymphocyte predominant Hodgkin's disease had IgH and Vkappa4 gene rearrangements, which supports the conclusion that this disease results from a proliferation of neoplastic B cells. The IgH and kappa and/or lambda gene rearrangements seen in H/R-S cells isolated from classic Hodgkin's disease (mixed cellularity-type and nodular sclerosis-type) support the theory that these cells derive from B lineage cells at various stages of differentiation. To our knowledge, this is first time that lambda gene rearrangements have been detected in H/R-S cells.

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Accession: 010757964

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PMID: 10439838

DOI: 10.1136/mp.52.1.37

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