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Homeobox gene expression and mutation in cervical carcinoma cells

, : Homeobox gene expression and mutation in cervical carcinoma cells. Cancer Science 94(5): 437-441, May

An association between deregulation of homeobox (HOX) gene expression and oncogenic transformation has been recently reported in human tumors. In this study, we investigated HOX gene expression and mutation in cervical carcinoma cells. Using reverse transcription-PCR, 11 human cervical carcinoma cell lines and 14 normal cervical tissue samples were examined for mRNA expression of the 39 class I HOX genes. DNA samples from 11 cell lines were tested for mutations in exons 1 and 2 of the HOXA10 and A13 genes using overlapping primer pairs which also cover intron 1 of these genes. HOXA1, B2, B4, C5, C10 and D13 genes were expressed in 8, 7, 9, 9, 9 and 11 of 11 cervical carcinoma cell lines, respectively, but not in any of the normal cervical tissues. HOXA9, A11, A13, B5, C4, D3 and D9 genes were expressed in all cell lines and normal tissues. In contrast, 13 of 39 HOX genes were silent in all materials examined. Single-strand conformational polymorphism and sequence analysis revealed a C insertion after base 1042 and/or a G to C substitution at base 1113 in intron 1 of the HOXA13 gene in 4 of 11 cell lines, however, neither deletions nor mutations were detected in exons 1 and 2 of the HOX A10 and A13 genes. Our data suggest that the expression of HOXA1, B2, B4, C5, C10 and D13 genes might be involved in the process leading to the transformation of normal cervical cells.

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Accession: 010758507

PMID: 12824890

DOI: 10.1111/j.1349-7006.2003.tb01461.x

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Niederberger, C., 2015: Re: shorter anogenital distance correlates with undescended testis: a detailed genital anthropometric analysis in human newborns. Journal of Urology 192(1): 181-182

Blaxter, K. L., 1975: The technical possibilities for animal production from grassland. The last 5 items were presented at a symposium held on 26 September 1974 to mark the 25th anniversary of the Grassland Research Institute at Hurley.

Laverov N.P.; Zhamoyda A.I.; Shaposhnikov G.N., 1982: Centennial of VSEGEI. Sovetskaya Geologiya 1982(3): 8-26

Hill W.T.; Morrie R.G.; Hagegeorge C.C., 1971: Ore controls and related sedimentary features at the Flat Gap Mine, Treadway, Tennessee. Economic Geology and the Bulletin of the Society of Economic Geologists: , Pages 748-756. 1971.

Wesolowski, J.; Nowicki, A., 1978: Use in surgery of an ultrasound device measuring the blood flow based on Doppler's effect. Polski Przeglad Chirurgiczny 50(1): 41-48

Gudinova, L.G.; Kalashnik, N.A.; Gamzikova, O.I.; Antipova, G.P.; Kachur, O.T., 1983: Variation in and combining ability for indices of the primary root system of spring bread wheat in Siberia. In a study of 7 varieties and 10 F1 intervarietal hybrids obtained from topcrosses in which Saratovskaya 29 and Atlas 66 were the testers used with 5 maternal varieties, quantitative characters of the primary root system were controlled by additiv...

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Moraes, L.A.H. de; Souza, E.L.S. de; Becker, R.F.P.; Braun, J., 1999: Chemical control of citrus leafminer Phyllocnistis citrella Stainton, 1856. Studies were conducted in Rio Grande do Sul, Brazil, in a citrus nursery of orange (var. 'Valencia') during 1997 to determine the effect of various insecticides for the control of citrus leafminer, Phyllocnistis citrella. Insecticides te...

Power, A. P., 1966: The Free Trade Area Agreement-implications for Irish agriculture. The Free Trade Agreement was concluded by the UK and Irish governments on December 14, 1965, and came into force on July 1, 1966. It aims at the elimination by July 1975 of customs duties and quota restrictions on a wide range of goods moving betw...

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