+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Human foamy virus Bel 1 transactivator/estrogen receptor fusion proteins allow inducible transactivation of both human foamy virus promoters

Human foamy virus Bel 1 transactivator/estrogen receptor fusion proteins allow inducible transactivation of both human foamy virus promoters

Virus Genes 21(3): 209-213

ISSN/ISBN: 0920-8569

PMID: 11129637

DOI: 10.1023/a:1008143615032

Recombinant plasmids that express human foamy virus (HFV) Bel 1 transactivator and human estrogen receptor (ER) fusion proteins were constructed. The HFV bel 1 gene was inserted up- and downstream of the ER gene. Recombinant Bel 1-ER and ER-Bel 1 fusion proteins were expressed in eukaryotic cells. In the absence of estrogen, the ER moiety of the fusion proteins suppressed Bel 1-mediated transactivation as measured in CAT reporter gene-based transactivation assays. However, transactivation of the HFV LTR and the HFV internal promoter by Bel 1-ER and ER-Bel 1 fusion proteins was recovered in the presence of estrogen. Thus, the transactivation function of the Bel 1 moiety of the chimeric Bel 1-ER fusion proteins can be efficiently, specifically, and intentionally activated and inactivated by simply adding the low-molecular weight effector estrogen.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 010765866

Download citation: RISBibTeXText

Related references

Isolement d'un nouveau rétrovirus foamy chez le cheval : l'EFV (equine foamy virus) - Isolation of a new foamy virus : the equine foamy virus. Virologie (Montrouge) 4(3): 248-249, 2000

Infectious proviral clones of chimpanzee foamy virus (SFVcpz) generated by long PCR reveal close functional relatedness to human foamy virus. Virology 214(2): 685-689, 1995

Transactivation of human immunodeficiency virus type 1 long terminal repeat-directed gene expression by the human foamy virus bel1 protein requires a specific DNA sequence. Journal of Virology 66(5): 3236-3240, 1992

Expression of the human foamy virus bel-1 transactivator in insect cells. Virus Research 30(1): 89-95, 1993

The transcriptional transactivator of human foamy virus maps to the bel 1 genomic region. Proceedings of the National Academy of Sciences of the United States of America 88(3): 941-945, 1991

Human foamy virus: further characterization, seroepidemiology, and relationship to chimpanzee foamy viruses. Journal of Infectious Diseases 137(4): 421-427, 1978

Antigenic relationship of human foamy virus to the simian foamy viruses. Infection and Immunity 20(1): 69-72, 1978

Regulation of gene expression by human foamy virus and potentials of foamy viral vectors. Stem Cells 15(Suppl 1): 141-147, 1997

Important role of N108 residue in binding of bovine foamy virus transactivator Tas to viral promoters. Virology Journal 13: 117, 2016

Residues R(199)H(200) of prototype foamy virus transactivator Bel1 contribute to its binding with LTR and IP promoters but not its nuclear localization. Virology 449: 215-223, 2014

Lysine residues K66, K109, and K110 in the bovine foamy virus transactivator protein are required for transactivation and viral replication. Virologica Sinica 31(2): 142-149, 2016

The gene expression of human foamy virus does not require a post-transcriptional transactivator. Virology 204(1): 409-413, 1994

BEL-1 transactivator responsive sequences in the long terminal repeat of human foamy virus. Virology 196(1): 256-268, 1993

Isolation, cloning, and sequencing of simian foamy viruses from chimpanzees (SFVcpz) : high mology to human foamy virus (HFV). Virology (New York) 201(2): 187-199, 1994

Isolation, cloning, and sequencing of simian foamy viruses from chimpanzees (SFVcpz): high homology to human foamy virus (HFV). Virology 201(2): 187-199, 1994