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Human serum albumin production in silkworm Bombyx mori fourth instar larvae



Human serum albumin production in silkworm Bombyx mori fourth instar larvae



Journal of Insect Biotechnology & Sericology 70(3): 183-188



Recombinant human serum albumin (rHSA) was produced using the recombinant Bombyx mori nucleopolyhedrovirus (rNPV) vector in silkworm larvae. rHSA was purified by ion exchange chromatography, affinity chromatography, and gel filtration chromatography from the whole-body homogenate of infected 4th instar larvae. No impurities were detected in the purified rHSA by reverse-phase chromatography and SDS-polyacrylamide gel electrophoresis. We obtained 3.48 mg of mature rHSA (removed preprosequence) from 24 4th instar larvae on day 4 postinfection (1X102 PFU/larva). These results suggest that efficient rHSA mass production is possible using the rNPV vector and B. mori 4th instar larvae.

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