Humoral immune response in Yersinia enterocolitica O:3: Infected Balb/c mice
De-Medeiros, B.-Maria-Machado; Malmegrim, K.-Cristina-Ribeiro; De-Queiroz, C.-Arantes; Cangiani, E.-Elena
Revista de Ciencias Farmaceuticas 21(1): 45-55
Balb/c mice were experimentally infected by oral or intravenous route with Yersinia enterocolitica O:3 for evaluation of their humoral immune response. A group of normal mice was used as control. Five mice from each group were bled by heart puncture and their spleens were removed on the 3rd, 7th, 14th, 21st and 28th day after infection. Immunoglobulin-secreting spleen cells were detected by the isotype-specific protein A plaque assay. The presence of specific anti-Yersinia antibodies was determined in mouse serum by ELISA. In the group infected by the intragastric route there was a reduction of immunoglobuling-secreting spleen cells compared to control during the first week after infection; the animals did not develop anti-Yersinia antibodies. In the group infected by the intravenous route there was an increase in the cells secreting immunoglobulins of all isotypes, with the following peaks of maximum activation: IgM on the 3rd day, IgG2a and IgG3 on the 7th day and IgA, IgG1 and IgG2b on the 14th day after infection. Specific antibodies of the IgG and IgM isotype were detected in the sera of these animals. Previous Balb/c mouse infection with Y. enterocolitica by the oral route did not change the patterns of humoral response to sheep red blood cells by these animals. We conclude that the immune humoral response differs between the animals inoculated intravenously or orally, with a short-term immunosupression being observed only in the latter group.