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IFN-gamma secretion by CD8T cells inhibits allergen-induced airway eosinophilia but not late airway responses


IFN-gamma secretion by CD8T cells inhibits allergen-induced airway eosinophilia but not late airway responses



Journal of Allergy and Clinical Immunology 109(5): 803-809



ISSN/ISBN: 0091-6749

PMID: 11994704

DOI: 10.1067/mai.2002.123233

Background: CD8+ T cells can suppress allergen-induced late airway responses (LARs) and airway inflammation. Objective: To test the hypothesis that the suppression of LARs and airway eosinophilia by CD8+ T cells is IFN-gamma mediated, we tested the effects of adoptively transferred CD8+ T cells, in which IFN-gamma synthesis was inhibited by an antisense (AS) oligodeoxynucleotide (ODN), on the airway responses of a rat model of allergic asthma. Methods: CD8+ T cells were harvested from the cervical lymph nodes of ovalbumin (OVA)-sensitized Brown Norway rats for administration to other actively sensitized syngeneic rats. CD8+ T cells (2 X 106) were incubated for 6 hours with 2 mumol/L AS ODN or sense ODN and were injected intraperitoneally into recipients; inhibition of IFN-gamma expression in vitro by AS ODN was shown by means of flow cytometry. Two days later, rats were challenged with aerosolized OVA. Results: OVA-induced LAR and bronchoalveolar lavage (BAL) fluid eosinophilia were suppressed by sense ODN-treated CD8+ T cells. IFN-gamma expression in BAL cells was elevated in these animals. IFN-gamma expression in BAL cells was at control levels in recipients of AS ODN-treated CD8+ cells, confirming the success of the AS treatment in vivo. BAL eosinophilia was also largely restored in the AS ODN treatment group. In contrast, the CD8+ T cell-induced suppression of the LAR was not significantly affected by AS ODN pretreatment. Conclusions: These results indicate that CD8+ T cells inhibit airway eosinophilia through secretion of IFN-gamma but may suppress the LAR by means of other mechanisms.

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Accession: 010776794

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