Identification of 1-adenine DNA adducts in workers occupationally exposed to styrene

Koskinen, M.; Vodicka, P.; Hemminki, K.

Journal of Occupational and Environmental Medicine 43(8): 694-700

2001


ISSN/ISBN: 1076-2752
PMID: 11515252
DOI: 10.1097/00043764-200108000-00007
Accession: 010781040

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Abstract
Styrene is an extensively used industrial chemical that has been classified as a possible human carcinogen. The possible carcinogenicity may be related to the covalent DNA binding properties of styrene 7,8-oxide, a major metabolite of styrene. We have developed a sensitive and a highly specific phosphorus-32-postlabeling method for the determination of 1-styrene 7,8-oxide-adenine DNA adducts. These adducts were analyzed in white blood cells from workers exposed to styrene at mean level of 76.2 mg/m3. Three of nine exposed workers showed adducts above the detection limit; the mean was 0.79 +/- 0.14 1-styrene 7,8-oxide-adenine DNA adducts/10(9) nucleotides. None of the 11 control cells showed adducts above the detection limit, which was 0.4 adducts/10(9) nucleotides. The results show a potential of 1-adenine DNA adducts for predicting risks in the workers exposed to styrene.