+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of the differentiation-associated Na+/PI transporter as a novel vesicular glutamate transporter expressed in a distinct set of glutamatergic synapses



Identification of the differentiation-associated Na+/PI transporter as a novel vesicular glutamate transporter expressed in a distinct set of glutamatergic synapses



Journal of Neuroscience 22(1): 142-155



Glutamate transport into synaptic vesicles is a prerequisite for its regulated neurosecretion. Here we functionally identify a second isoform of the vesicular glutamate transporter (VGLUT2) that was previously identified as a plasma membrane Na+-dependent inorganic phosphate transporter (differentiation-associated Na+/P(I) transporter). Studies using intracellular vesicles from transiently transfected PC12 cells indicate that uptake by VGLUT2 is highly selective for glutamate, is H+ dependent, and requires Cl- ion. Both the vesicular membrane potential (Deltapsi) and the proton gradient (DeltapH) are important driving forces for vesicular glutamate accumulation under physiological Cl- concentrations. Using an antibody specific for VGLUT2, we also find that this protein is enriched on synaptic vesicles and selective for a distinct class of glutamatergic nerve terminals. The pathway-specific, complementary expression of two different vesicular glutamate transporters suggests functional diversity in the regulation of vesicular release at excitatory synapses. Together, the two isoforms may account for the uptake of glutamate by synaptic vesicles from all central glutamatergic neurons.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 010788842

Download citation: RISBibTeXText

PMID: 11756497


Related references

Identification of the Differentiation-Associated Na + /P I Transporter as a Novel Vesicular Glutamate Transporter Expressed in a Distinct Set of Glutamatergic Synapses. The Journal of Neuroscience 22(1): 142-155, 2002

Vesicular glutamate transporter 1 and vesicular glutamate transporter 2 synapses on cholinergic neurons in the sublenticular gray of the rat basal forebrain: a double-label electron microscopic study. Neuroscience 164(4): 1721-1731, 2010

Identification of endophilins 1 and 3 as selective binding partners for VGLUT1 and their co-localization in neocortical glutamatergic synapses: implications for vesicular glutamate transporter trafficking and excitatory vesicle formation. Cellular and Molecular Neurobiology 26(4-6): 679-693, 2006

Identification of differentiation-associated brain-specific phosphate transporter as a second vesicular glutamate transporter (VGLUT2). Journal of Neuroscience 21(22): Rc182, 2001

Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neurons. Nature 407(6801): 189-194, 2000

Changes in vesicular glutamate transporter 2, vesicular GABA transporter and vesicular acetylcholine transporter labeling of sacrocaudal motoneurons in the spastic rat. Experimental Neurology 197(2): 407-419, 2005

Differentiation-associated Na+-dependent inorganic phosphate cotransporter is a vesicular glutamate transporter in endocrine glutamatergic systems. Cell Structure & Function 27(4): 285, 2002

Differentiation-associated Na+-dependent inorganic phosphate cotransporter (DNPI) is a vesicular glutamate transporter in endocrine glutamatergic systems. Journal of Biological Chemistry 276(46): 43400-6, 2001

Evidence for a glutamatergic amacrine cell vesicular glutamate transporter 3 is expressed in a subset of amacrine cells in the rodent retina. ARVO Annual Meeting Abstract Search & Program Planner : Abstract No 2069, 2003

Vesicular glutamate transporter and glutamatergic signaling. Tanpakushitsu Kakusan Koso. Protein, Nucleic Acid, Enzyme 48(13): 1816-1823, 2003

Vesicular glutamate transporter-3 in the rodent brain: vesicular colocalization with vesicular γ-aminobutyric acid transporter. Journal of Comparative Neurology 521(13): 3042-3056, 2014

The existence of a second vesicular glutamate transporter specifies subpopulations of glutamatergic neurons. Journal of Neuroscience 21(22): Rc181, 2001

The expression of vesicular glutamate transporter 3 and vesicular monoamine transporter 2 induced by brain-derived neurotrophic factor in dorsal root ganglion neurons in vitro. Brain Research Bulletin 100: 93-106, 2014

Vesicular glutamate transporter expression in supraoptic neurones suggests a glutamatergic phenotype. Journal of Neuroendocrinology 18(4): 253-265, 2006

Glutamate vesicular transporter bNPI and putative excitatory amino acid vesicular transporter dNPI show differential distribution in neurons projecting to cerebellar cortex in rat. Society for Neuroscience Abstracts 27(1): 1506, 2001