+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Implementation of a flow-cytometric assay for rapid detection of HPA-1-compatible platelet donors under routine conditions

Implementation of a flow-cytometric assay for rapid detection of HPA-1-compatible platelet donors under routine conditions

Infusion Therapy and Transfusion Medicine 29(1): 6-10

Background: Antibodies against the HPA-1a platelet antigen are a frequent cause of alloimmune thrombocytopenia. For diagnosis and transfusion requirements, blood banks should provide a number of HPA-1-typed platelet donors. Material and Methods: We evaluated a serological, flow-cytometric HPA-1 typing assay allowing for the differentiation of the HPA-1a and -1b variant of the platelet glycoprotein GPIIIa (ADIAflo(R) Platelet HPA-1). Results: Of 172 donors tested, 6 HPA-1b-homozygous individuals were identified. These results were confirmed by SSP-PCR genotyping. 120 donors were tested HPA-1a-homozygous. The test also distinguishes HPA-1a-homozygous from HPA-1a/1b-heterozygous platelets (n=46). Donors with a result near the cut-off value between HPA-1a-homozygous and HPA-1a/1b-heterozygous were retyped by SSP-PCR. In this group, we observed one case of mistyping by the serological procedure. Conclusion: The ADIAflo Platelet HPA-1 typing assay has been successfully implemented in our daily routine for rapid detection of HPA-1-compatible platelet donors. Results near the cut-off value distinguishing HPA-1a-homozygous from HPA-1a/1b-heterozygous samples should be confirmed by genotyping.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 010805188

Download citation: RISBibTeXText

DOI: 10.1159/000057079

Related references

A rapid flow cytometric screening test for X-linked lymphoproliferative disease due to XIAP deficiency. Cytometry. Part B Clinical Cytometry 76(5): 334-344, 2009

A rapid and reliable enzyme immunoassay PCR-based screening method to identify EBV-carrying gastric carcinomas. Modern Pathology 15(8): 870-877, 2002

A rapid flow cytometric method to monitor plasma concentrations of GP IIb-IIIa antagonists in the clinic. Blood 84(10 Suppl. 1): 321A, 1994

Rapid multiplex reverse transcription-PCR typing of influenza A and B virus, and subtyping of influenza A virus into H1, 2, 3, 5, 7, 9, N1 (human), N1 (animal), N2, and N7, including typing of novel swine origin influenza A (H1N1) virus, during the 2009 outbreak in Milwaukee, Wisconsin. Journal of Clinical Microbiology 47(9): 2772-2778, 2009

Development and characteristics of resistance in the susceptible WHO/SRS house fly (Musca domestica) strain subjected to selection with beta-cypermethrin. Pesticide Science 45(4): 335-349, 1995

Flow cytometric assay of pinocytosis Correlation with membrane ruffling and metastatic potential in the Dunning R-3327 rat prostatic adenocarcinoma model. Cytometry. 14(7): 826-831, 1993

Open Minds on Open Borders. Journal of International Migration and Integration 14(3): 401-417, 2013

Antiphospholipid antibody syndrome: new insights on thrombogenic mechanisms. American Journal of the Medical Sciences 316(2): 142-151, 1998

Determination of heparin-induced IgG antibody in heparin-induced thrombocytopenia type II. European Journal of Clinical Investigation 29(3): 232-237, 1999

A rapid and sensitive fiber optic immunoassay for microorganisms. Abstracts of Papers American Chemical Society 206(1-2): ANYL 65, 1993

Detection of platelet-drived growth factor-AB in patients serum with hepatic cirrhosis before and after splenectomy. Journal of Gastroenterology and Hepatology 14(Suppl A): A357, 1999

Detection of bacterial cells by impedance spectra via fluidic electrodes in a microfluidic device. Lab on a Chip 10(12): 1557-1560, 2010

Detection of platelet antimembrane antibodies using an elisa method. 1985