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Improved selectivity for high-performance liquid chromatographic determination of clonazepam in plasma of epileptic patients



Improved selectivity for high-performance liquid chromatographic determination of clonazepam in plasma of epileptic patients



Journal of Chromatography B 719(1-2): 227-233



We report a high-performance liquid chromatography method for clonazepam determination in plasma. The use of a synthetic silica-based stationary phase markedly improved clonazepam resolution compared to standard reversed-phase columns. A liquid-liquid extraction was used, associated with reversed-phase chromatography, gradient elution and ultraviolet detection. Accuracy and precision were satisfactory at therapeutic concentrations. Selectivity was studied for benzodiazepines or other antiepileptic drugs, with particular attention to newly marketed drugs i.e., gabapentine and vigabatrin. No interfering substance was evidenced. Under the conditions described, it was possible to quantify clonazepam at nanogram level even when carbamazepine was present at therapeutic concentrations. rights reserved.

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Accession: 010808117

Download citation: RISBibTeXText

PMID: 9869385

DOI: 10.1016/s0378-4347(98)00391-0


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