Section 11
Chapter 10,819

In vivo regulation of the beta-myosin heavy chain gene in soleus muscle of suspended and weight-bearing rats

Giger, J.M.; Haddad, F.; Qin, A.X.; Baldwin, K.M.; Baldwin, K.M.

American Journal of Physiology. Cell Physiology 278(6): C1153-C1161


ISSN/ISBN: 0363-6143
PMID: 10837343
DOI: 10.1152/ajpcell.2000.278.6.c1153
Accession: 010818005

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In the weight-bearing hindlimb soleus muscle of the rat, approximately 90% of muscle fibers express the beta-myosin heavy chain (beta-MHC) isoform protein. Hindlimb suspension (HS) causes the MHC isoform population to shift from beta toward the fast MHC isoforms. Our aim was to establish a model to test the hypothesis that this shift in expression is transcriptionally regulated through specific cis elements of the beta-MHC promoter. With the use of a direct gene transfer approach, we determined the activity of different length beta-MHC promoter fragments, linked to a firefly luciferase reporter gene, in soleus muscle of control and HS rats. In weight-bearing rats, the relative luciferase activity of the longest beta-promoter fragment (-3500 bp) was threefold higher than the shorter promoter constructs, which suggests that an enhancer sequence is present in the upstream promoter region. After 1 wk of HS, the reporter activities of the -3500-, -914-, and -408-bp promoter constructs were significantly reduced ( approximately 40%), compared with the control muscles. However, using the -215-bp construct, no differences in promoter activity were observed between HS and control muscles, which indicates that the response to HS in the rodent appears to be regulated within the -408 and -215 bp of the promoter.

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