+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

In-house low-resolution X-ray crystallography

In-house low-resolution X-ray crystallography

Acta Crystallographica Section D Biological Crystallography 56(10): 1304-1311

It is demonstrated that standard in-house protein crystal X-ray diffraction apparatus can be used to measure very low resolution reflections with only a few modifications. The apparatus and modifications are described in detail and tested on two different macromolecular crystal samples: lysozyme and the 30S ribosomal subunit. Contrast-variation measurements on tetragonal hen egg-white lysozyme demonstrate the potential usefulness of the apparatus in providing accurate data for the determination of macromolecular envelopes. In contrast, the measurement of very low resolution diffraction from crystals of the 30S ribosome subunit illustrates how in-house facilities can provide data from small weakly diffracting crystals of a very large macromolecule.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 010818461

Download citation: RISBibTeXText

PMID: 10998626

DOI: 10.1107/s0907444900008982

Related references

Sub-atomic resolution X-ray crystallography and neutron crystallography: promise, challenges and potential. Iucrj 2(Pt 4): 464-474, 2015

Energy optimization of a regular macromolecular crystallography beamline for ultra-high-resolution crystallography. Journal of Synchrotron Radiation 22(1): 172-174, 2014

Protonation-state determination in proteins using high-resolution X-ray crystallography: effects of resolution and completeness. Acta Crystallographica. Section D, Biological Crystallography 68(Pt 7): 800-809, 2012

House Joint Resolution 309, House Joint Resolution 331, to permit the use of oleomargarine by the armed forces. 1948

Sub-atomic Resolution Crystal Structure of Cholesterol Oxidase: What Atomic Resolution Crystallography Reveals about Enzyme Mechanism and the Role of the FAD Cofactor in Redox Activity. Journal of Molecular Biology 326(5): 35-50, 2003

Low-resolution crystallography is coming of age. Structure 13(2): 171-172, 2005

Developments in low-resolution biological X-ray crystallography. F1000 Biology Reports 2: 80, 2010

Studies of borate minerals; 1, X-ray crystallography of colemanite; 2, X-ray crystallography of inyoite and meyerhofferite; x-ray and morphological crystallography of 2CaO.3B (sub 2) O (sub 3) .9H (sub 2) O; 3 (and Clark, Joan Robinson, and Evans, Howard Tasker, Jr.). The crystal structure of colemanite, CaB (sub 3) O (sub 4) (OH) (sub 3) .H (sub 2) O. American Mineralogist 38(3-4): 411-415, 1953

Approaching atomic resolution in crystallography of ribosomes. Annual Review of Biophysics and Biomolecular Structure 21: 77-93, 1992

Resolution of phase ambiguity in macromolecular crystallography. Methods in Enzymology 115: 90-112, 1985

High-resolution crystallography and drug design. Journal of Molecular Recognition 18(3): 196-202, 2005

Resolution of structural heterogeneity in dynamic crystallography. Acta Crystallographica. Section D, Biological Crystallography 69(Pt 6): 946-959, 2013

Assessment of resolution in biological electron crystallography. Ultramicroscopy. 47(1-3): 256-265, 1992