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Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA



Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA



Chinese Medical Journal 116(8): 1262-1264



Objective: To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases. Methods: Vero-E6 cells was transfected with siRNA before SARS virus infection and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells. Results: Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect. Conclusion: siRNA may be effective in inhibiting SARS-associated coronavirus replication.

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Accession: 010846948

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PMID: 12935424


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