Inhibitory effects of low-density lipoproteins from men with type II diabetes on endothelium-dependent relaxation
McNeill, K.L.; Fontana, L.; Russell-Jones, D.L.; Rajman, I.; Ritter, J.M.; Chowienczyk, P.J.
Journal of the American College of Cardiology 35(6): 1622-1627
ISSN/ISBN: 0735-1097 PMID: 10807469 DOI: 10.1016/s0735-1097(00)00607-0
OBJECTIVES: The object of the present study is to determine whether native (n) low-density lipoprotein (LDL) isolated from men with type II diabetes and abnormal endothelial function inhibits endothelium-dependent relaxation more than n-LDL isolated from nondiabetic control subjects. BACKGROUND: Endothelium-dependent vasodilation is impaired in men with type II diabetes and this may result from qualitative rather than quantitative abnormalities of LDL. METHODS: Forearm blood flow responses to brachial artery infusions of acetylcholine (endothelium-dependent vasodilator) and nitroprusside (endothelium-independent vasodilator) were measured in 10 men with uncomplicated type II diabetes and 10 nondiabetic men of similar age and with similar plasma concentrations of LDL cholesterol. Native LDL was isolated by discontinuous density gradient ultracentrifugation using EDTA to prevent oxidation. Preconstricted rabbit aortic ring bioassay was used to determine inhibitory properties of n-LDL on endothelium-dependent relaxation by measuring relaxation to acetylcholine (and nitroprusside) in the presence and absence of n-LDL. RESULTS: Forearm blood flow responses to acetylcholine but not nitroprusside were significantly impaired (p < 0.01) in diabetic men compared with control subjects. Native LDL (10 and 100 mug protein/ml) from diabetic men inhibited relaxation to acetylcholine by 13.9 +- 4.8% and 61.9 +- 7.8% (mean inhibition for all doses +- SE), respectively, whereas n-LDL from control subjects inhibited relaxation by 7.3 +- 3.0% and 23.9 +- 5.7% (p < 0.01 for a difference between diabetic and control n-LDL). Relaxation to nitroprusside was not significantly inhibited by n-LDL. CONCLUSIONS: A qualitative abnormality of LDL may account for endothelial dysfunction in men with type II diabetes.