+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Inputs from intrinsic primary afferent neurons to nitric oxide synthase-immunoreactive neurons in the myenteric plexus of guinea pig ileum



Inputs from intrinsic primary afferent neurons to nitric oxide synthase-immunoreactive neurons in the myenteric plexus of guinea pig ileum



Cell & Tissue Research 299(1): 1-8



Nitric oxide synthase (NOS) immunoreactivity occurs in two groups of neurons in the guinea pig small intestine: descending interneurons that are also immunoreactive for choline acetyltransferase (ChAT), and inhibitory motor neurons that lack ChAT immunoreactivity. Interneurons that are involved in local reflexes would be expected to have inputs from intrinsic primary afferent (sensory) neurons, most of which are calbindin-immunoreactive. We examined this possibility using triple staining for NOS, ChAT and calbindin immunoreactivity and investigated the relationships between calbindin-immunoreactive varicosities and the cell bodies of NOS-immunoreactive neurons, using high-resolution confocal microscopy and electron microscopy. By confocal microscopy, we found that the cell bodies of ChAT/NOS interneurons received 84+-23 (mean +- SD) direct appositions from calbindin-immunoreactive varicosities and that the cell bodies of NOS-inhibitory motor neurons received 82+-20 appositions. Electron-microscopic examination of the relations of 265-calbindin-immunoreactive varicosities, at distances within the resolution of the confocal microscope (300 nm), to 30 NOS-immunoreactive nerve cells indicated that 84% formed close contacts or synapses and 16% were separated from neurons by thin glial cell processes. Thus, each NOS-immunoreactive nerve cell receives about 70 synaptic inputs or close contacts from the calbindin-immunoreactive varicosities of intrinsic primary afferent neurons. It is concluded that there are monosynaptic reflex connections in which intrinsic primary afferent neurons synapse directly with motor neurons and di- or poly-synaptic reflexes in which ChAT- and NOS-immunoreactive neurons are interneurons, interposed between intrinsic primary afferent neurons and NOS-inhibitory neurons.

(PDF emailed within 0-6 h: $19.90)

Accession: 010855376

Download citation: RISBibTeXText

PMID: 10654065

DOI: 10.1007/s004410050001


Related references

5-HT activates intestinal intrinsic primary afferent neurons via 5-HT3 receptors in the guinea pig myenteric plexus. Society for Neuroscience Abstracts 24(1-2): 879, 1998

W1351 In Vitro Electrochemical Detection of Nitric Oxide Produced By Inhibitory Motor Neurons in the Myenteric Plexus of the Guinea Pig Ileum. Gastroenterology 134(4): A-686, 2008

Analysis of connections between nitric oxide synthase neurons in the myenteric plexus of the guinea-pig small intestine. Journal Of Neurocytology. 24(4): 257-263, 1995

An ultrastructural study of serotoninergic inputs to neurons of the myenteric plexus of the guinea pig ileum. Society for Neuroscience Abstracts 18(1-2): 487, 1992

The terminals of myenteric intrinsic primary afferent neurons of the guinea-pig ileum are excited by 5-hydroxytryptamine acting at 5-hydroxytryptamine-3 receptors. Neuroscience 101(2): 459-469, 7 November, 2000

P2X receptors mediate synaptic transmission from descending interneurons, but not from intrinsic primary afferent neurons, to inhibitory motor neurons in the guinea-pig ileum. Gastroenterology 118(4 Suppl 2 Part 1): AGA A877, 2000

Properties of synaptic inputs from myenteric neurons innervating submucosal S neurons in guinea pig ileum. American Journal of Physiology. Gastrointestinal and Liver Physiology 278(2): G273-G280, 2000

Distribution of nitric oxide synthase containing neurons in the rectal myenteric plexus and anal canal. Morphologic evidence that nitric oxide mediates the rectoanal inhibitory reflex. Diseases of the Colon and Rectum 37(4): 350-357, 1994

Electrochemical monitoring of nitric oxide released by myenteric neurons of the guinea pig ileum. Neurogastroenterology and Motility 20(11): 1243-1250, 2008

Extrinsic denervation increases NADPH diaphorase staining but not citrulline production by nitric oxide synthase in myenteric plexus of guinea pig ileum. Society for Neuroscience Abstracts 19(1-3): 963, 1993

Electrical stimulation of the mucosa excites primary afferent neurons of the guinea-pig myenteric plexus. Journal of the Autonomic Nervous System 65(2-3): 129, 1997

Actions of ethanol on longitudinal muscle motor neurons and sensory neurons in the myenteric plexus of isolated guinea-pig ileum. Journal of Physiology (Cambridge) 495P(0): 55P-56P, 1996

Distribution of nitric oxide synthase containing neurons in the rectal myenteric plexus and anal canal. Diseases of the Colon & Rectum 37(4): 350-357, 1994

Ascending choline acetyltransferase and descending nitric oxide synthase immunoreactive neurones of the myenteric plexus project to the mucosa of the guinea pig gastric corpus. Neuroscience Letters. 241(1): 61-64,. 23, 1998

Developmental changes of nitric oxide synthase positive neurons in myenteric plexus of small intestine in calf. Veterinary Science in China 37(6): 529-533, 2007