EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Integrin-independent tyrosine phosphorylation of p125(fak) in human platelets stimulated by collagen


Integrin-independent tyrosine phosphorylation of p125(fak) in human platelets stimulated by collagen



Journal of Biological Chemistry 276(5): 3167-3174



ISSN/ISBN: 0021-9258

PMID: 11110790

DOI: 10.1074/jbc.m007186200

Collagen fibers or a glycoprotein VI-specific collagen-related peptide (CRP-XL) stimulated tyrosine phosphorylation of the focal adhesion kinase, p125(fak) (FAK), in human platelets. An integrin alpha(2)beta(1)-specific triple-helical peptide ligand, containing the sequence GFOGER (single-letter nomenclature, O = Hyp) was without effect. Antibodies to the alpha(2) and beta(1) integrin subunits did not inhibit platelet FAK tyrosine phosphorylation caused by either collagen fibers or CRP-XL. Tyrosine phosphorylation of FAK caused by CRP-XL or thrombin, but not that caused by collagen fibers, was partially inhibited by GR144053F, an antagonist of integrin alpha(IIb)beta(3). The intracellular Ca(2+) chelator, BAPTA, and the protein kinase C inhibitor, Ro31-8220, were each highly effective inhibitors of the FAK tyrosine phosphorylation caused by collagen or CRP-XL. These data suggest that, in human platelets, 1) occupation or clustering of the integrin alpha(2)beta(1) is neither sufficient nor necessary for activation of FAK, 2) the fibrinogen receptor alpha(IIb)beta(3) is not required for activation of FAK by collagen fibers, and 3) both intracellular Ca(2+) and protein kinase C activity are essential intermediaries of FAK activation.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010859599

Download citation: RISBibTeXText

Related references

A collagen-like peptide stimulates tyrosine phosphorylation of syk and phospholipase C gamma2 in platelets independent of the integrin alpha2beta1. Blood 89(4): 1235-1242, 1997

Tyrosine phosphorylation and relocation of SHIP are integrin-mediated in thrombin-stimulated human blood platelets. Journal of Biological Chemistry 272(43): 26857-26863, 1997

Evidence against a direct role of the integrin α2β1 in collagen-induced tyrosine phosphorylation in human platelets. Febs Journal 267(7): 2088-2097, 2000

Evidence against a direct role of the integrin a2b1 in collagen-induced tyrosine phosphorylation in human platelets. European Journal of Biochemistry 267(7): 88-97, 2000

Evidence against a direct role of the integrin alpha2beta1 in collagen-induced tyrosine phosphorylation in human platelets. European Journal of Biochemistry 267(7): 2088-2097, 2000

Protein-tyrosine phosphorylation and p72syk activation in human platelets stimulated with collagen is dependent upon glycoprotein Ia/IIa and actin polymerization. Thrombosis and Haemostasis 75(4): 648-654, 1996

Participation of tyrosine phosphorylation in cytoskeletal reorganization, alpha(IIb)beta(3) integrin receptor activation, and aspirin-insensitive mechanisms of thrombin-stimulated human platelets. Circulation 102(16): 1924-1930, 2000

Tyrosine phosphorylation of the Fc receptor c-chain in collagen-stimulated platelets. The Journal of Biological Chemistry 271: 095-9, 1996

An integrin independent phosphorylation of a novel related adhesion focal tyrosine kinase in platelets. Blood 88(10 Suppl 1 Part 1-2): 23A, 1996

Tyrosine phosphorylation of the Fc receptor gamma-chain in collagen-stimulated platelets. Journal of Biological Chemistry 271(30): 18095-18099, 1996

Differential regulation of protein-tyrosine phosphatases by integrin aIIbb3 through cytoskeletal reorganization and tyrosine phosphorylation in human platelets. The Journal of Biological Chemistry 270: 927-34, 1995

Clustering of integrin alphaIIb-beta3 differently regulates tyrosine phosphorylation of pp72syk, PLCgamma2 and pp125FAK in concanavalin A-stimulated platelets. Thrombosis and Haemostasis 81(1): 124-130, 1999

Differential regulation of protein-tyrosine phosphatases by integrin alpha IIb beta 3 through cytoskeletal reorganization and tyrosine phosphorylation in human platelets. Journal of Biological Chemistry 270(20): 11927-11934, 1995

Glycoprotein VI/Fc receptor gamma chain-independent tyrosine phosphorylation and activation of murine platelets by collagen. Biochemical Journal 383(Pt. 3): 581-588, 2004

Collagen, convulxin, and thrombin stimulate aggregation-independent tyrosine phosphorylation of CD31 in platelets. Evidence for the involvement of Src family kinases. Journal of Biological Chemistry 275(35): 27339-27347, 2000