+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Interleukin-3 and interferon beta cooperate to induce differentiation of monocytes into dendritic cells with potent helper T-cell stimulatory properties



Interleukin-3 and interferon beta cooperate to induce differentiation of monocytes into dendritic cells with potent helper T-cell stimulatory properties



Blood 99(3): 993-998



It was observed that interferon beta (IFN-beta) prevents the down-regulation of the interleukin-3 receptor alpha chain (IL-3Ralpha), which spontaneously occurs during culture of human monocytes. The functionality of IL-3R was demonstrated by the fact that IL-3 rescued IFN-beta-treated monocytes from apoptosis. Monocytes cultured in the presence of IFN-beta and IL-3 acquire a dendritic morphology and express high levels of HLA antigen class I and class II and costimulatory molecules. When stimulated by either lipopolysaccharide or fibroblasts expressing CD40 ligand (CD40L) transfectants, dendritic cells (DCs) generated in IFN-beta and IL-3 secreted high levels of IL-6, IL-8, and tumor necrosis factor-alpha but low levels of IL-12 in comparison with DCs generated in IL-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF). In mixed leukocyte culture, IL-3-IFN-beta DCs induced a vigorous proliferative response of allogeneic cord blood T cells and elicited the production of high levels of IFN-gamma and IL-5 by naive adult CD4+ T cells. Finally, IL-3-IFN-beta DCs were found to produce much higher levels of IFN-alpha than IL-4-GM-CSF DCs in response to Poly (I:C) but not to influenza virus. It was concluded that monocytes cultured in the presence of IL-3 and IFN-beta differentiate into DCs with potent helper T-cell stimulatory capacity despite their low secretion of IL-12.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 010867865

Download citation: RISBibTeXText

PMID: 11807004


Related references

Activated CD8+ T lymphocytes induce differentiation of monocytes to dendritic cells and restore the stimulatory capacity of interleukin 10-treated antigen-presenting cells. Cancer Research 62(17): 5065-5068, 2002

Plasmacytoid dendritic cells induce plasma cell differentiation through type I interferon and interleukin 6. Immunity 19(2): 225-234, 2003

Human dendritic cells induce the differentiation of interleukin-21-producing T follicular helper-like cells through interleukin-12. Immunity 31(1): 158-169, 2009

BCG stimulated dendritic cells induce an interleukin-10 producing T-cell population with no T helper 1 or T helper 2 bias in vitro. Immunology 121(2): 276-282, 2007

Regulation of differentiation and functional properties of monocytes and monocyte-derived dendritic cells by interferon beta in multiple sclerosis. Multiple Sclerosis 10(5): 499-506, 2004

T helper/inducer (CD4+) cells prestimulated with PPD induce monocytes to produce interleukin-1 beta. Journal of Leukocyte Biology 49(6): 542-547, 1991

Interferon-beta increases the stimulatory capacity of monocyte-derived dendritic cells to induce IL-13, IL-5 and IL-10 in autologous T-cells. Journal of Neuroimmunology 123(1-2): 160-169, 2002

Freshly isolated Peyer's patch, but not spleen, dendritic cells produce interleukin 10 and induce the differentiation of T helper type 2 cells. Journal of Experimental Medicine 190(2): 229-239, 1999

Interleukin 6 (interferon beta 2) and interferon alpha/beta present in postendotoxin serum induce differentiation of murine M1 myeloid leukemia cells. Experimental Hematology 17(11): 1063-1066, 1989

Thyroid papillary carcinoma Tumor cells release factors able to recruit dendritic cells and to induce dendritic cell differentiation from peripheral blood CD14+ monocytes. Virchows Archiv 439(3): 383, 2001

Recombinant human interferon-alpha and interferon-beta interleukin-6 but not interferon-gamma induce megakaryocytic differentiation of the acute megakaryoblastic leukaemia blast cells. Pathology Research & Practice 189(6-7): 712, 1993

Interferon gamma and interleukin 2 potent activation factors that cooperate to induce macrophage resistance to infection. Journal of Leukocyte Biology 42(4): 400, 1987

Interferon-alpha and interleukin-6 in SLE serum induce the differentiation and maturation of dendritic cells derived from CD34+ hematopoietic precursor cells. Cytokine 50(2): 195-203, 2010

Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-gamma production by T helper 1 cells. European Journal of Immunology 26(3): 659-668, 1996

Monocytes Primed With Interferon-Differentiate Into Maturation-Arrested Regulatory Dendritic Cells That Induce T Cell Hyporesponsiveness. Transplantation 86(Suppl.): 507-508, 2008