+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Involvement of mitogen-activated protein kinases in the signal transduction pathway of bone marrow-derived macrophage activation in response to in vitro treatment with thymosin alpha 1



Involvement of mitogen-activated protein kinases in the signal transduction pathway of bone marrow-derived macrophage activation in response to in vitro treatment with thymosin alpha 1



International Immunopharmacology 2(1): 47-58



Thymosin alpha 1 (Talpha1), a 28-amino acid, acidic thymic peptide, is a promising natural biological response modifier (BRM), which augments and regulates the immune network and is thought to be immunostimulatory also. Recently, we have reported the ability of Talpha1 to activate macrophages to tumoricidal state. In the present investigation, the activation of the p42/44 MAP kinase (MAPK)/c-Jun NH2 terminal kinase (JNK) pathway in response to in vitro treatment with Talpha1 in murine bone marrow-derived macrophages (BMDMs) has been demonstrated. The activation and expression of phospho-p42/44 MAPK was dose as well as time dependent with maximum expression occurring at 5-15 min following stimulation with 100 ng/ml of Talpha1. The expression of phospho-p42/44 MAPK was inhibited by the MAPK inhibitor, PD98059, pertussis toxin (PTX), tyrosine kinase inhibitor-genistein and PI3K inhibitor-wortmannin. Talpha1-induced BMDM tumoricidal functions like the production of NO and TNF-alpha, the key mediator molecules of macrophage cytotoxicity, were also inhibited by the MAPK inhibitor, PD98059, in a dose-dependent manner. These observations suggest that p42/44 MAPK activation is one of the essential signaling events triggered by Talpha1 and may be responsible for the in vitro activation of BMDMs.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010882858

Download citation: RISBibTeXText

PMID: 11789669

DOI: 10.1016/s1567-5769(01)00139-4


Related references

Activation of mitogen-activated protein kinases cellular signal transduction pathway in mammalian cells induced by silicon carbide nanowires. Biomaterials 31(31): 7856-7862, 2010

Effects of hydroxyethyl starch 130/0.4 on P38 mitogen-activated protein kinases signal transduction pathway. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 21(5): 270-273, 2010

Following in vitro activation of mitogen-activated protein kinases by mass spectrometry and tryptic peptide analysis: purifying fully activated p38 mitogen-activated protein kinase alpha. Analytical Biochemistry 336(1): 1-10, 2004

Following in vitro activation of mitogen-activated protein kinases by mass spectrometry and tryptic peptide analysis: purifying fully activated p38 mitogen-activated protein kinase alpha. Analytical Biochemistry 336(1): 1-10, 2005

Cholera toxin activation of mitogen-activated protein kinases Induction of signal transduction pathways distinct from CFTR activation. Gastroenterology 122(4 Suppl 1): A 542, 2002

Porphyromonas gingivalis gingipains trigger a proinflammatory response in human monocyte-derived macrophages through the p38α mitogen-activated protein kinase signal transduction pathway. Toxins 2(3): 341-352, 2010

Citreorosein inhibits production of proinflammatory cytokines by blocking mitogen activated protein kinases, nuclear factor-κB and activator protein-1 activation in mouse bone marrow-derived mast cells. Biological and Pharmaceutical Bulletin 35(6): 938-945, 2012

Involvement of the p38 mitogen-activated protein kinase signal transduction pathway in burns-induced lung injury. Chinese Medical Journal 118(4): 329-332, 2005

Involvement of mitogen-activated protein kinase activation in the signal-transduction pathways of the soya bean oxidative burst. Biochemical Journal 355(Pt 3): 795-803, 2001

Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases. Journal of Biological Chemistry 267(36): 25628-25631, 1992

Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-? B in mouse peritoneal macrophage-derived foam cells. 2011

Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells. Chinese Journal of Integrative Medicine 18(5): 391-394, 2012

Extracellular Nef protein activates signal transduction pathway from Ras to mitogen-activated protein kinase cascades that leads to activation of human immunodeficiency virus from latency. Aids Research and Human Retroviruses 18(6): 461-467, 2002

Fc gamma R-dependent mitogen-activated protein kinase activation in leukocytes: a common signal transduction event necessary for expression of TNF-alpha and early activation genes. Journal of Experimental Medicine 184(3): 1027-1035, 1996

Luteolin-7-O-glucoside suppresses leukotriene C(4) production and degranulation by inhibiting the phosphorylation of mitogen activated protein kinases and phospholipase Cγ1 in activated mouse bone marrow-derived mast cells. Biological and Pharmaceutical Bulletin 34(7): 1032-1036, 2011