EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Irradiation induces increase of adhesion molecules and accumulation of beta2-integrin-expressing cells in humans



Irradiation induces increase of adhesion molecules and accumulation of beta2-integrin-expressing cells in humans



International Journal of Radiation Oncology, Biology, Physics 45(2): 475-481



Purpose: The purpose of our investigation was to describe the dose- and time-dependent histomorphologic alterations of the irradiated tissue, the composition of the infiltrate, and the expression patterns of various adhesion molecules. Methods and Materials: We analyzed immunohistochemically alterations in oral mucosa in 13 head and neck cancer patients before radiotherapy and with 30 Gy and 60 Gy. All had oral mucosa irradiation, with a final dose of 60 Gy using conventional fractionation. Snap-frozen specimens were stained using the indirect immunperoxidase technique. Histomorphology was studied in paraffin-embedded sections. In addition, we determined the clinical degree of oral mucositis. Results: Histomorphologic evaluation showed no vascular damage. Irradiation caused a steep increase of beta2-integrin-bearing cells (p < 0.01), whereas the percentage of beta1-integrin-positive cells remained at low levels. Additionally we found an increase in the expression of endothelial intercellular adhesion molecule-1 (ICAM-1) (p < 0.01) and E-selectin (p < 0.05), while endothelial vascular cell adhesion molecule-1 (VCAM-1) expression remained at very low levels. Conclusion: Our findings indicate that in radiation-induced oral mucositis there is no marked vascular damage until the end of radiotherapy. For recruitment of leukocytes, beta2 is more involved than beta1. Pharmaceuticals that block leukocyte adhesion to E-selectin or ICAM-1 may prevent radiation-mediated inflammation in oral mucosa.

(PDF emailed within 1 workday: $29.90)

Accession: 010885623

Download citation: RISBibTeXText

PMID: 10487574



Related references

The effects of intensive, moderate and downhill treadmill running on human blood lymphocytes expressing the adhesion/activation molecules CD54 (ICAM-1), CD18 (beta2 integrin) and CD53. European Journal of Applied Physiology 97(1): 109-121, 2006

IL-2 induces beta2-integrin adhesion via a wortmannin/LY294002-sensitive, rapamycin-resistant pathway. Phosphorylation of a 125-kilodalton protein correlates with induction of adhesion, but not mitogenesis. Journal of Immunology 157(12): 5350-5358, 1996

Antagonism of isoprenaline- and salbutamol-stimulated cyclic AMP accumulation by beta2-antagonists in CHO-K1 cells expressing the human beta2-adrenoceptor. British Journal of Pharmacology 136(Proceedings Supplement): 51P, June, 2002

Expression of cell adhesion molecules and their beta1 and beta2 integrin ligands in human liver peliosis. Pathology, Research and Practice 201(7): 503-511, 2005

Phosphoinositide 3-OH kinase activates the beta2 integrin adhesion pathway and induces membrane recruitment of cytohesin-1. Journal of Biological Chemistry 273(24): 14853-14861, 1998

Medium-chain, triglyceride-containing lipid emulsions increase human neutrophil beta2 integrin expression, adhesion, and degranulation. Jpen. Journal of Parenteral and Enteral Nutrition 24(4): 228-233, 2000

Determining beta2-integrin and intercellular adhesion molecule 1 binding kinetics in tumor cell adhesion to leukocytes and endothelial cells by a gas-driven micropipette assay. Journal of Biological Chemistry 286(40): 34777-34787, 2011

Interleukin-2 induces beta2-integrin-dependent signal transduction involving the focal adhesion kinase-related protein B (fakB). Proceedings of the National Academy of Sciences of the United States of America 95(12): 6959-6964, 1998

Disruption of integrin-dependent adhesion and survival of endothelial cells by recombinant adenovirus expressing isolated beta integrin cytoplasmic domains. Gene Therapy 7(15): 1292-1303, 2000

Staphylococcal enterotoxin-A-induced in-vitro adhesion of HL-60 cells to endothelial cells involves both selectin and integrin families of cell adhesion molecules. Scandinavian Journal of Immunology 48(2): 127-135, 1998

Quantification and kinetic study of cell-cell adhesion between Jurkat cells expressing alpha4beta1 integrin and S2 cells expressing VCAM-1 by two-color flow cytometry. Molecular Biology of the Cell 13(Supplement): 349a, 2002

CD44 cross-linking induces integrin-mediated adhesion and transendothelial migration in breast cancer cell line by up-regulation of LFA-1 (alpha L beta2) and VLA-4 (alpha4beta1). Experimental Cell Research 304(1): 116-126, 2005

Beta2-integrin adhesion caused by eotaxin but not IL-5 is blocked by PDE-4 inhibition and beta2-adrenoceptor activation in human eosinophils. Pulmonary Pharmacology & Therapeutics 17(2): 73-79, 2004

Construction and characterization of modulators of gene expression elements for Mel-CAM and beta2 integrin adhesion molecules by gene specific-random retroviral libraries. Proceedings of the American Association for Cancer Research Annual Meeting 39: 59, March, 1998