+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Irreversible binding of biologically reduced 2,4,6-trinitrotoluene to soil

Irreversible binding of biologically reduced 2,4,6-trinitrotoluene to soil

Environmental toxicology and chemistry 18(11): 2416-2423

To analyze the fate of 2,4,6-trinitrotoluene (TNT) and its reduction products, a TNT-contaminated soil (350 mg TNT/kg dry soil) was spiked with [ring-UL-14C]TNT and treated in a laboratory slurry reactor. During an anaerobic/aerobic treatment, the total radioactivity measured in the supernatant and methanolic soil extracts decreased to 2%. The decrease corresponded to an increase of strongly bound radioactivity to the soil. Throughout the whole treatment process, mineralization of TNT was not observed. During the reductive process, unidentified polar substances increased to a maximum amount of 23.2% of the total radioactivity on the day after the start of the experiment. After the end of the anaerobic phase, still 9.7% of the radioactivity was found in this fraction. Only during the aerobic phase did the polar substances disappear completely. The irreversible character of the binding of the reduced metabolites of TNT to the soil was indicated by the failure of desorption even under rigorous and long-term extraction conditions. A significant release of radioactivity could be measured only by using high concentrations of HCl (5 N) or EDTA (12.5% and 5.9%, respectively). However, in none of the extracts were TNT or any reduced metabolites detected by HPLC and 14C-radiocounting. Size-exclusion chromatography of humic acids extracted from the treated soil indicated that the metabolites of TNT were evenly bound to the complete range of molecular size of the humic acids.

(PDF emailed within 0-6 h: $19.90)

Accession: 010885715

Download citation: RISBibTeXText

DOI: 10.1002/etc.5620181106

Related references

Solid-state nitrogen-15 nuclear magnetic resonance analysis of biologically reduced 2,4,6-trinitrotoluene in a soil slurry remediation. Journal of Environmental Quality 30(2): 403-410, 2001

Transformation and binding of 2,4,6-trinitrotoluene in soil amended with activated carbon. Ecological significance of the interactions among clay minerals, organic matter and soil biota 3rd Symposium on Soil Mineral Organic Matter Microorganism Interactions and Ecosystem Health, Naples Capri, Italy, 22-26 May 2000: 157-168, 2002

Binding of 2,4,6-trinitrotoluene and its degradation products in a soil organic matter two-phase system. Journal of Environmental Quality 30(6): 2053-2061, 2002

Binding of 2,4,6-trinitrotoluene and its degradation products in a soil organic matter two-phase system. Journal of environmental quality 30(6): 2053-2061, 2001

Binding of 2,4,6-trinitrotoluene, aniline, and nitrobenzene to dissolved and particulate soil organic matter. Environmental Science and Technology 38(11): 3074-3080, 2004

The implications of irreversible binding of activated foreign compounds to macro molecules the irreversible binding of 4 di methylamino phenol to rat kidney proteins. Naunyn-Schmiedeberg's Archives of Pharmacology 297(SUPPL II): R21, 1977

Degradation of trinitrotoluene in contaminated soils as affected by its initial concentrations and its binding to soil organic matter fractions. Journal of Environmental Science and Health. Part A, Toxic/Hazardous Substances & Environmental Engineering 43(4): 348-356, 2008

Reduced and controlled surface binding of biologically active molecules. Official Gazette of the United States Patent & Trademark Office Patents 1177(3): 1704, Aug 15, 1995

Irreversible binding of chlorophenols to soil and its impact on bioavailability. Journal of Environmental Engineering New York 123(5): 506-513, 1997

Irreversible binding of PAHs and PCBs by soil organic matter. Abstracts of Papers American Chemical Society, National Meeting: 1-2, Pages GEOC 069. 1998., 1998

Probes for narcotic receptor mediated phenomena. 10. Irreversible ligands to opioid receptors based on biologically potent endoethenooripavines. Reversible binding of fit to mu and delta opioid receptors. Neuropeptides 5(1-3): 229-232, 1984

Reversible sorption and irreversible binding of naphthalene and a-naphthol to soil: elucidation of processes. Environmental Science and Technology 30: 05-11, 1996

Irreversible binding of carbon 14 carbon tetra chloride metabolites to reduced phosphor pyridine nucleotides in vivo and in vitro. Naunyn-Schmiedeberg's Archives of Pharmacology 293(SUPPL): R65, 1976

Reversible sorption and irreversible binding of naphthalene and alpha-Naphthol to soil: Elucidation of processes. Environmental Science and Technology 30(4): 1205-1211, 1996

Kinetics of irreversible enzyme inhibition: the Tsou plot in irreversible binding co-operativity. Journal of Theoretical Biology 70(4): 461-465, 1978