+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Islet amyloid polypeptide inhibits glucagon release and exerts a dual action on insulin release from isolated islets



Islet amyloid polypeptide inhibits glucagon release and exerts a dual action on insulin release from isolated islets



Regulatory Peptides 111(1-3): 55-60, 28 March



We have studied the influence of a wide concentration range of islet amyloid polypeptide (IAPP) on both glucagon and insulin release stimulated by various types of secretagogues. In an islet incubation medium devoid of glucose, the rate of glucagon release being high, we observed a marked suppressive action by low concentrations of IAPP, 10-10 and 10-8 M, on glucagon release. Similarly, glucagon release stimulated by L-arginine, the cholinergic agonist carbachol, or the phosphodiesterase inhibitor isobutylmethyl xanthine (IBMX), an activator of the cyclic AMP system, was inhibited by IAPP in the 10-10 and 10-8 M concentration range. Moreover, basal glucagon release at 7 and 10 mM glucose was suppressed by IAPP. In contrast, IAPP exerted a dual action on insulin release. Hence, low concentrations of IAPP brought about a modest increase of basal insulin secretion at 7 mM glucose and also of insulin release stimulated by carbachol. High concentrations of IAPP, however, inhibited insulin release stimulated by glucose (10 and 16.7 mM), IBMX, carbachol and L-arginine. In conclusion, our data suggest that IAPP has complex effects on islet hormone secretion serving as an inhibitor of glucagon release and having a dual action on insulin secretion exerting mainly a negative feedback on stimulated and a positive feedback on basal insulin release.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010889074

Download citation: RISBibTeXText

PMID: 12609749

DOI: 10.1016/s0167-0115(02)00252-5


Related references

Influence of islet amyloid polypeptide and the 8-37 fragment of islet amyloid polypeptide on insulin release from perifused rat islets. Diabetes 42(2): 330-335, 1993

Inhibitory action of islet amyloid polypeptide and calcitonin gene-related peptide on release of insulin from the isolated perfused rat pancreas. Pancreas 6(4): 459-463, 1991

Islet amyloid polypeptide inhibits glucose-stimulated insulin secretion from isolated rat pancreatic islets. Biochemical and Biophysical Research Communications 160(2): 961-967, 1989

Characteristics of insulin and glucagon release from the perfused pancreas, intact isolated islets, and dispersed islet cells. Hormone Research 24(1): 62-72, 1986

Neurotensin inhibits glucose but not glucagon-induced insulin and somatostatin release in isolated islets. Diabete & Metabolisme 5(4): 275-277, 1979

Stimulation of insulin release in isolated rat islets by gastric inhibitory polypeptide in physiological concentrations and its relation to islet cyclic amp content. Diabetologia 28(11): 857-861, 1985

Dual action of phentolamine on insulin release from isolated rat islets. Journal of Clinical Investigation 53(6): 8A, 1974

Islet amyloid polypeptide tonally inhibits b-, a-, and d-cell secretion in isolated rat pancreatic islets. American Journal of Physiology 276(1): 9-E24, 1999

Simultaneous capillary electrophoresis competitive immunoassay for insulin, glucagon, and islet amyloid polypeptide secretion from mouse islets of Langerhans. Journal of Chromatography. A 1218(26): 4059-4064, 2011

Effects of islet amyloid polypeptide on insulin secretion from isolated rat pancreatic islets. Digestion 59(3): 253, May-June, 1998

Islet amyloid polypeptide tonally inhibits beta-, alpha-, and delta-cell secretion in isolated rat pancreatic islets. American Journal of Physiology 276(1 Pt 1): E19-E24, 1999

Effect of islet amyloid polypeptide on somatostatin inhibition of insulin secretion from isolated rat pancreatic islets. Regulatory Peptides. 72(1): 61-67,. 26, 1997

Islet amyloid polypeptide potentiates somatostatin-induced inhibition of insulin secretion from isolated rat pancreatic islets. Digestion 57(4): 275, 1996

Salivary peptide P-C potentiates insulin release and inhibits glucagon release from isolated perfused pancreas of the diabetic GK rat. Japanese Journal of Pharmacology 67(1): 15-20, 1995