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Isolation of U(VI) reduction-deficient mutants of Shewanella putrefaciens



Isolation of U(VI) reduction-deficient mutants of Shewanella putrefaciens



FEMS Microbiology Letters 184(2): 143-148



A U(VI) reduction-deficient mutant (Urr) screening technique was developed and combined with chemical mutagenesis procedures to identify a Urr mutant of Shewanella putrefaciens strain 200. The Urr mutant lacked the ability to grow anaerobically on U(VI) and NO(2)(-), yet retained the ability to grow anaerobically on eight other compounds as terminal electron acceptor. All 11 members of previously isolated sets of Fe(III) and Mn(IV) reduction-deficient mutants of S. putrefaciens 200 displayed Urr-positive phenotypes with the Urr screen and were capable of anaerobic growth on U(VI). This is the first reported isolation of a respiratory mutant that is unable to grow anaerobically on U(VI) as terminal electron acceptor.

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Accession: 010892737

Download citation: RISBibTeXText

PMID: 10713412

DOI: 10.1111/j.1574-6968.2000.tb09005.x


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