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Kinetic deoxyribose degradation assay and its application in assessing the antioxidant activities of phenolic compounds in a Fenton-type reaction system

Kinetic deoxyribose degradation assay and its application in assessing the antioxidant activities of phenolic compounds in a Fenton-type reaction system

Analytica Chimica Acta 478(1): 129-137, 12 February

Pro-oxidant properties of phenolic antioxidants, which are derived from their iron recycling reactivity, render the traditional deoxyribose degradation assay invalid to assess the hydroxyl radical-scavenging activity in Fenton-type reaction systems. In the present paper, we studied in detail the interactions between iron and phenolic compounds, and established a kinetic deoxyribose method by taking advantage of the distinct difference between the completion time of Fenton reaction and that of the iron-reducing process. With the newly established kinetic method, we investigated the effects of phenolics on hydroxyl radical formation in a Fenton-type system and determined successfully the second rate constants of hydroxyl radical-scavenging reactions. The site-specific and non-site-specific hydroxyl radical-scavenging ability suggested that both direct hydroxyl radical-scavenging potency and iron-chelating capacity accounted for their inhibitory effects on deoxyribose oxidation degradation. This method, more simple, time saving, and applicative than the traditional deoxyribose assay, produces as accurate results (RSD < 0.05, with dynamic range from 7.5 to 575 muM) as typical methods, such as radiolysis technology, and may be of significance in evaluating and screen the hydroxyl radical-scavenging antioxidants.

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Accession: 010899807

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DOI: 10.1016/s0003-2670(02)01435-6

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