+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Knowledge-based system for method development of chiral separations with capillary electrophoresis using highly-sulphated cyclodextrins



Knowledge-based system for method development of chiral separations with capillary electrophoresis using highly-sulphated cyclodextrins



Journal of Pharmaceutical & Biomedical Analysis 27(3-4): 515-529, 15 January



Method development for chiral separations is not easy because it requires experience and many experimental possibilities can be chosen. In order to help the analyst, a knowledge-based system (KBS) for the rapid determination of experimental parameters, which allow a baseline separation of enantiomers, has been developed. On the basis of own laboratory knowledge, completed with literature data, rules were defined and a KBS was built. Five different techniques are considered in this KBS. This paper describes the capillary electrophoresis (CE) section, in which a strategy has been defined based on the use of highly-sulfated cyclodextrins as chiral selectors. A structured representation of the knowledge and its implementation in Toolbook software is presented.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010902088

Download citation: RISBibTeXText

PMID: 11755753

DOI: 10.1016/s0731-7085(01)00574-x


Related references

Rapid screening for chiral separations by short-end injection capillary electrophoresis using highly sulfated cyclodextrins as chiral selectors. Electrophoresis 22(15): 3203-3215, 2001

Robustness testing of chiral separations by capillary electrophoresis using highly-sulfated cyclodextrins. Journal of Chromatography. A 1007(1-2): 165-177, 2003

Chiral separations of lobeline analogs using high performance capillary electrophoresis and derivatized cyclodextrins as chiral additives. Abstracts of Papers American Chemical Society 213(1-3): MEDI 232, 1997

Chiral separations by capillary zone electrophoresis using functionalized cyclodextrins. Abstracts of Papers American Chemical Society 210(1-2): ANYL 11, 1995

Selectivity in capillary electrophoresis:  application to chiral separations with cyclodextrins. Analytical Chemistry 69(3): 385-392, 1997

Quantitative approach for the screening of cyclodextrins by nuclear magnetic resonance spectroscopy in support of chiral separations in liquid chromatography and capillary electrophoresis enantioseparation of norgestrel with alpha-, beta- and gamma-cyclodextrins. Journal of Chromatography. a 961(2): 257-276, 2002

Application of sulfated cyclodextrins to chiral separations by capillary zone electrophoresis. Analytical Chemistry 68(8): 1360-1368, 1996

Comprehensive strategy for chiral separations using sulfated cyclodextrins in capillary electrophoresis. Chirality 15(8): 709-723, 2003

Recent innovations in the use of charged cyclodextrins in capillary electrophoresis for chiral separations in pharmaceutical analysis. Electrophoresis 21(15): 3220-3239, 2000

Evaluation of chiral ionic liquids as additives to cyclodextrins for enantiomeric separations by capillary electrophoresis. Journal of Chromatography. a 1155(2): 134-141, 2007

Systematic screening approach for chiral separations of basic compounds by capillary electrophoresis with modified cyclodextrins. Journal of Pharmaceutical and Biomedical Analysis 19(5): 679-694, 1999

Enantioseparation of pharmaceutical compounds by multiplexed capillary electrophoresis using highly sulphated alpha-, beta- and gamma-cyclodextrins. Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 875(1): 248-253, 2008

Method development strategies for the enantioseparation of drugs by capillary electrophoresis using cyclodextrins as chiral additives. Electrophoresis 19(16-17): 2834-2840, 1998

Chiral separations of underivatized arylpropionic acids by capillary zone electrophoresis with various cyclodextrins: acidity and inclusion constant determinations. Journal of Chromatography A 735(1-2): 311-320, 1996

Optimization of enantiomeric separations for basic compounds in capillary zone electrophoresis using neutral and anionic cyclodextrins as chiral selectors. Biomedical Chromatography 9(6): 265-266, 1995