+ Translate
+ Most Popular
Sobre os generos Lopadusa Stal e Bothrocoris Mayr (Hemiptera, Pentatomidae, Pentatomini)
RFEF pilot plant for inactivation of Escherichia coli in apple juice
Calpionella alpina Lorenz als "Leitfossil"
Sooretama: The Atlantic Rain Forest of Brazil. Francis Dov Por
Novel approaches in myeloma therapy
Preliminary Notes on the Study of Mitochondria During the 1st Stages of the Destruction of Cadavers
A preliminary study of the biology of lichee stink bug, Tessaratoma papulosa Drury, and its control
The status of the Sabu Island python - Die Situation des Savu-Pythons
Randomised trial of intravenous immunoglobulin as prophylaxis against infection in plateau-phase multiple myeloma. the UK Group for Immunoglobulin Replacement therapy in Multiple Myeloma
Conflicts between children
Clinical presentations in neuropsychiatry
Method of suturing a perforated ulcer of the stomach and duodenum
Identification of mechanical asphyxiation in cases of attempted masking of the homicide
Effects of endogenous ascorbate on oxidation, oxygenation, and toxicokinetics of cell-free modified hemoglobin after exchange transfusion in rat and guinea pig
Is it warm? Is it real? Or just low spatial frequency?
Additions and corrections to the list of Korean Heteroptera
Factors contributing to the maintenance of oligotrophy in an alpine glacial moraine lake (Wallowa lake) in Northeast Oregon
Classification, paleoecology, and biostratigraphy of crinoids from the Stull Shale (Late Pennsylvanian) of Nebraska, Kansas, and Iowa
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Neue Formen der Bauchsammler aus Turkmenien (Hymenoptera, Apoidea)
Atypische Hühnerpest und Newcastle-Krankheit. Ein Identifizierungsversuch
Parasitellus fucorum in the debris of Apis mellifera - a risk of confusion with Tropilaelaps spp
Applying the Technology Acceptance Model to the introduction of healthcare information systems
Ein akarologischer Nachruf für den "Roland"
Mutation research on crop plants in Argentina

Lens regeneration in Xenopus is not a mere repeat of lens development, with respect to crystallin gene expression

Lens regeneration in Xenopus is not a mere repeat of lens development, with respect to crystallin gene expression

Differentiation; Research in Biological Diversity 64(3): 143-149

ISSN/ISBN: 0301-4681

PMID: 10234811

DOI: 10.1046/j.1432-0436.1999.6430143.x

The spatio-temporal expression of three crystallin genes (alpha A, beta B1 and gamma) in lenses of Xenopus laevis was studied by in situ hybridization to compare the process of lens formation in embryonic development with that of lens regeneration from cornea that occurs in the tadpole. During embryonic lens development, all three crystallin transcripts were initially detected at the same stage of lens placode formation, and subsequently their signals became restricted to the presumptive lens fiber region. At later stages, the three crystallin genes were expressed in primary and secondary lens fibers, but not in lens epithelium. During lens regeneration, alpha A- and beta B1-crystallin signals were first detected in the presumptive lens fiber region of the lens vesicle. The expression of gamma-crystallin, however, appeared later than the other two crystallin genes and was detected only in morphologically discernible lens fibers. In the later stages of lens regeneration, expression of these crystallins was observed only in the lens fiber region, similar to embryonic lens development. These results reveal that lens regeneration from the inner layer of the outer cornea is not simply a repetition of embryonic lens development, when examined at the level of crystallin gene transcription.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 010916899

Download citation: RISBibTeXText

Related references

Requirement for betaB1-crystallin promoter of Xenopus laevis in embryonic lens development and lens regeneration. Development Growth and Differentiation 47(3): 131-140, 2005

The expression of αA- and βB1-crystallin during normal development and regeneration, and proteomic analysis for the regenerating lens in Xenopus laevis. Molecular Vision 17: 768-778, 2011

Pax-6 and Prox 1 expression during lens regeneration from Cynops iris and Xenopus cornea: evidence for a genetic program common to embryonic lens development. Differentiation; Research in Biological Diversity 65(3): 141-149, 1999

Gene expression profiles of lens regeneration and development in Xenopus laevis. Developmental Dynamics: An Official Publication of the American Association of Anatomists 238(9): 2340-2356, 2009

Xenopus gamma-crystallin gene expression: evidence that the gamma-crystallin gene family is transcribed in lens and nonlens tissues. Molecular and Cellular Biology 14(2): 1355-1363, 1994

Reiteration frequency of delta-crystallin DNA in lens and non-lens tissues of chick embryos. delta-Crystallin gene is not amplified during lens cell differentiation. Journal of Biological Chemistry 251(14): 4294-4298, 1976

Lens specific expression of the cat gene promoted by 5 flanking sequences of the murine alpha a crystallin gene in explanted chicken lens epithelia. Journal Of Cellular Biochemistry Supplementt B: 155, 1985

Chicken lens delta-crystallin gene expression and methylation in several non-lens tissues. Nucleic Acids Research 11(9): 2513-2527, 1983

Structure and expression of the lens fiber cell specific beta 35 crystallin gene of the chicken eye lens. Journal of Cellular Biochemistry Suppl (9 Part B): 157, 1985

Lens-specific expression of the chloramphenicol acetyltransferase gene promoted by 5' flanking sequences of the murine alpha A-crystallin gene in explanted chicken lens epithelia. Proceedings of the National Academy of Sciences of the United States of America 82(8): 2334-2338, 1985

Immuno fluorescence studies of chick lens 1st important soluble crystallin and alpha crystallin antigens during lens morphogenesis and development. Ophthalmic Research 2(6): 344-357, 1971

Crystallin gene expression during rat lens development. European Journal of Biochemistry 183(1): 31-36, 1989

Lens Development and Crystallin Gene Expression. Progress in Molecular Biology and Translational Science 134: 129-167, 2015

Lens development and crystallin gene expression: many roles for Pax-6. Bioessays: News and Reviews in Molecular Cellular and Developmental Biology 18(8): 621-630, 1996

Conservation of gene expression during embryonic lens formation and cornea-lens transdifferentiation in Xenopus laevis. Developmental Dynamics: An Official Publication of the American Association of Anatomists 215(4): 308-318, 1999