Magnetite-alginate beads for purification of some starch degrading enzymes

Teotia, S.; Gupta, M.N.

Molecular Biotechnology 20(3): 231-237


ISSN/ISBN: 1073-6085
PMID: 11936253
DOI: 10.1385/mb:20:3:231
Accession: 010949638

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Starch degrading enzymes, viz., beta-amylase, glucoamylase, and pullulanase, were purified using magnetite-alginate beads. In each case, the enzyme activity was eluted by using 1.0 M maltose. beta-Amylase (sweet potato), glucoamylase (Aspergillus niger), and pullulanase (Bacillus acidopullulyticus) from their crude preparations were purified 37-, 31-, and 49-fold with 86, 87, and 95% activity recovery, respectively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed single band in each case.