+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Matrix Gla protein is differentially expressed during the deposition of a calcified matrix by vascular pericytes

Matrix Gla protein is differentially expressed during the deposition of a calcified matrix by vascular pericytes

FEBS Letters 487(2): 267-271, 29 December

PCR-based subtractive hybridisation was used to identify genes up-regulated when pericytes undergo osteogenic differentiation and deposit a calcified matrix. cDNA pools were generated from confluent pericytes and from pericyte cultures containing calcified nodules. A pericyte cDNA library was screened with the product of the subtraction procedure (calcified minus confluent cDNA) and the majority of the positive clones were identified as matrix Gla protein (MGP). Northern analysis and immunohistochemistry demonstrated that MGP was only expressed by pericytes in calcified nodules. Antibodies to MGP inhibited the deposition of a calcified matrix by pericytes, suggesting that MGP regulates both cell differentiation and calcification.

(PDF emailed within 0-6 h: $19.90)

Accession: 010960819

Download citation: RISBibTeXText

DOI: 10.1016/s0014-5793(00)02363-2

Related references

Matrix Gla protein is differentially expressed during the deposition of a calcified matrix by vascular pericytes. Febs Letters 487(2): 267-271, 2001

Identification of differentially expressed genes in human varicose veins: involvement of matrix gla protein in extracellular matrix remodeling. Journal of Vascular Research 44(6): 444-459, 2007

Incorporation of biomimetic matrix molecules in PEG hydrogels enhances matrix deposition and reduces load-induced loss of chondrocyte-secreted matrix. Journal of Biomedical Materials Research. Part A 97(3): 281-291, 2011

Sex steroids modulate human aortic smooth muscle cell matrix protein deposition and matrix metalloproteinase expression. Hypertension 46(5): 1129-1134, 2005

Deposition and resorption of calcified matrix in vitro by rat marrow cells. Cells & Materials 1(1): 3-16, 1991

The expression of cartilage oligomeric matrix protein, thrombospondin-1, bone sialoprotein and osteopontin in calcified and non-calcified arterial lesions. Biochemical Society Transactions 26(1): S3-S3, 2000

Novel conformation-specific antibodies against matrix gamma-carboxyglutamic acid (Gla) protein: undercarboxylated matrix Gla protein as marker for vascular calcification. Arteriosclerosis, Thrombosis, and Vascular Biology 25(8): 1629-1633, 2005

Smads differentially mediate vascular smooth muscle cell proliferation and matrix protein production. Circulation 104(17 Supplement): II 258-II 259, October 23, 2001

In vitro Ca deposition by rat matrix vesicles: is the membrane association of alkaline phosphatase essential for matrix vesicle-mediated calcium deposition?. International Journal of Biochemistry 25(12): 1737-1742, 1993

Expression of matrix metalloprotease-9 in vascular pericytes in human breast cancer. Laboratory Investigation; A Journal of Technical Methods and Pathology 77(4): 345-355, 1997

In vitro calcified matrix deposition by human osteoblasts onto a zinc-containing bioactive glass. European Cells & Materials 21: 59-72; Discussion 72, 2011

Matrix metalloproteinases regulate migration, proliferation, and death of vascular smooth muscle cells by degrading matrix and non-matrix substrates. Cardiovascular Research 69(3): 614-624, 2005

Cartilage oligomeric matrix protein (thrombospondin-5) is expressed by human vascular smooth muscle cells. Arteriosclerosis, Thrombosis, and Vascular Biology 21(1): 47-54, 2001

Matrix metalloproteinase 28, a novel matrix metalloproteinase, is constitutively expressed in human intervertebral disc tissue and is present in matrix of more degenerated discs. Arthritis Research & Therapy 11(6): R184-R184, 2010

The gastroliths of the crayfish as convenient sites for the study of mechanisms of matrix and mineral deposition in calcified structures. FRED PROC 19(1 Pt 1): 248, 1960