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Matrix methods for solving protein substructures of chlorine and sulfur from anomalous data



Matrix methods for solving protein substructures of chlorine and sulfur from anomalous data



Acta Crystallographica Section D Biological Crystallography 57(12): 1857-1862



The weak signal obtained from the anomalous scattering (at lambda=1.54 ANG) of naturally occurring elements such as sulfur, phosphorus and ordered solvent chloride ions is used to determine the atomic positions of these atoms. Two examples are discussed: the sulfur and chlorine substructure of tetragonal hen egg-white lysozyme and an oligonucleotide containing ten P atoms. The substructure of lysozyme was also solved from Cu Kalpha radiation data collected on a standard rotating-anode generator. The results presented here are an illustration of the power of the matrix methods, which are to be implemented in next distribution of the direct methods package CRUNCH.

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Accession: 010961207

Download citation: RISBibTeXText

PMID: 11717499

DOI: 10.1107/s0907444901016535


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