+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Melanin has a role in Ca2+ homeostasis in human melanocytes



Melanin has a role in Ca2+ homeostasis in human melanocytes



Pigment Cell Research 16(2): 127-132



We have examined whether melanin affects Ca2+ homeostasis in cultured normal human melanocytes. Intracellular Ca2+ concentrations ([Ca2+]i), were measured in four Caucasian and in three Negroid melanocyte cultures. Under resting conditions [Ca2+]i was around 100 nM in all cultures, but differences between cells within cultures were observed. All cultures responded to endothelin-1 (ET-1) with increases in [Ca2+]i and there were no differences between Caucasian and Negroid cultures. However, large differences in responses between cells within cultures were observed, indicating that melanocyte cultures are very heterogeneous. The addition of 2.5 mM CaCl2 to melanocytes kept in Ca2+-free medium resulted in rapid and transient increases in [Ca2+]i of up to 1500 nM. These increases were on average more than two times smaller in melanocyte cultures established from Negroid donors compared with Caucasian cultures. In addition, well melanized Caucasian melanocytes, cultured in the presence of 400 microM tyrosine and 10 mM NH4Cl, showed a reduced increase in cytoplasmic Ca2+ concentration upon the addition of extracellular Ca2+. The difference in maintaining Ca2+ homeostasis between poorly and well melanized melanocytes may be the result of the clearance of cytoplasmic Ca2+ into melanosomes and the greater capacity for this in the more pigmented melanocytes.

(PDF emailed within 0-6 h: $19.90)

Accession: 010971351

Download citation: RISBibTeXText

PMID: 12622789

DOI: 10.1034/j.1600-0749.2003.00018.x


Related references

Differential responses of human melanocytes with different melanin contents to UV blight and the role of the cAMP pathway in the UVB-induced melanogenesis. Photochemistry & Photobiology 63(SPEC ISSUE): 42S, 1996

Assessment of UV-induced DNA photoproducts and their repair in cultured human Melanocytes: Role of constitutive Melanin content and MC1R status. Pigment Cell Research 17(4): 438-438, 2004

Calcium homeostasis in human melanocytes: role of transient receptor potential melastatin 1 (TRPM1) and its regulation by ultraviolet light. American Journal of Physiology. Cell Physiology 297(3): C679-C687, 2009

The role of peroxidase vs. the role of tyrosinase in enzymatic conversion of tyrosine to melanin in melanocytes, mast cells and eosinophils. Journal of Investigative Dermatology 55(1): 1-12, 1970

Role of melanin pigment in the malignant transformation of melanocytes. Journal of Investigative Dermatology 94(4): 573, 1990

Changes in distribution pattern of cytoplasmic filaments in human melanocytes during uv mediated melanin pigmentation the role of the 100 angstrom filaments in the elongation of melanocytic dendrites and in the movement and transfer of melanosomes. Journal of Cell Biology 65(2): 481-488, 1975

Changes in Distribution Pattern of Cytoplasmic Filaments in Human Melanocytes during Ultraviolet-Mediated Melanin Pigmentation. The Role of the 100- Filaments in the Elongation of Melanocytic Dendrites and in the Movement and Transfer of Melanosomes. Journal of Cell Biology 65(2): 481-488, 1975

Role of melanin pigments in the response of melanocytes to solar irradiation. Pigment Cell Research 0(SUPPL 5): 62-63, 1996

A possible role for melanin precursors in regulating both pigmentation and proliferation of melanocytes. Progress in Clinical and Biological Research 256: 143-154, 1988

Cytoplasmic filaments in melanocytes their nature and role in melanin pigmentation. Riley, V (Ed ) Pigment Cell, Vol 3 Unique Properties Of Melanocytes Proceedings Of The 9th International Conference, Part Ii Houston, Tex , Usa, Jan 13-17, 1975 Xv+430p Illus S Karger: Basel, Switzerland; New York, N Y , Usa ISBN 3-8055-2371-8: 13-32, 1976

Changes in distribution pattern of cytoplasmic filaments in human melanocytes during ultraviolet-mediated melanin pigmentation. The role of the 100-Angstrom filaments in the elongation of melanocytic dendrites and in the movement and transfer of melanosomes. Journal of Cell Biology 65(2): 481-488, 1975

The role of peroxidase vs the role of tyrosinase in enzymatic conversion of tyrosine to melanin in melanocytes mast cells and eosinophils an auto radiographic histochemical study. Journal of Investigative Dermatology 55(1): 1-12, 1970

The melanocytes and melanin in human skin autografts. Plastic and Reconstructive Surgery 53(2): 200-207, 1974

Melanin from epidermal human melanocytes: study by pyrolytic GC/MS. Journal of the American Society for Mass Spectrometry 20(3): 464-468, 2009

Quantitative melanin determination in human cultured melanocytes. Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 182(6): 584-588, 1988