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Metabolite transport in isolated yeast mitochondria: Fumarate/malate and succinate/malate antiports



Metabolite transport in isolated yeast mitochondria: Fumarate/malate and succinate/malate antiports



FEBS Letters 462(3): 313-316, Dec 3



In this study, we investigated the metabolite permeability of isolated coupled Saccharomyces cerevisiae mitochondria. The occurrence of a fumarate/malate antiporter activity was shown. The activity differs from that of the dicarboxylate carrier (which catalyses the succinate/malate antiport) in (a) kinetics (Km and Vmax values are about 27 muM and 22 nmol min-1 mg protein-1 and 70 muM and 4 nmol min-1 mg protein-1, respectively), (b) sensitivity to inhibitors, (c) Ki for the competitive inhibitor phenylsuccinate and (d) pH profiles.

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Accession: 010977092

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DOI: 10.1016/s0014-5793(99)01535-5



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