+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Migration of langerhans cells and dermal dendritic cells in skin organ cultures: augmentation by TNF-alpha and IL-1beta



Migration of langerhans cells and dermal dendritic cells in skin organ cultures: augmentation by TNF-alpha and IL-1beta



Journal of Leukocyte Biology 66(3): 462-470



Migration from sites of antigen encounter to lymphoid organs is essential to the strong immunogenic function of dendritic cells (DC). In the skin, migration proceeds through dermal lymphatic vessels and is regulated in an incompletely understood way by inflammatory mediators. We studied the effects of tumor necrosis factor alpha (TNF-alpha) and interleukin-1beta (IL-1beta) in mouse skin organ cultures by direct enumeration of migrating DC and by immunohistochemistry. (1) Neutralizing antibodies to TNF-alpha and IL-1beta inhibited migration of DC, also in human skin explants (TNF-alpha). (2) TNF-alpha at low concentrations (50 U/mL) and IL-1beta (50-3000 U/mL) augmented migration to about 150% of spontaneous migration. (3) High concentrations of TNF-alpha (5000 U/mL) inhibited migration by approximately 50%. (4) DC migration from skin explants of TNF-alpha/lymphotoxin-alpha double-deficient mice and TNF-receptor type 1 and 2 double knock-out mice was not impaired. (5) TNF-alpha effects were neutralized by anti-IL-1beta, and vice versa. We conclude that in normal animals both TNF-alpha and IL-1beta are required for DC migration to occur. In the complete absence of one cytokine (TNF-alpha), however, backup mechanisms step in.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 010987597

Download citation: RISBibTeXText

PMID: 10496317


Related references

Migration of Langerhans cells and dermal dendritic cells in skin organ cultures: augmentation by Tnf- and Il-1. Journal of Leukocyte Biology 66(3): 462-470, 1999

Matrix metalloproteinases 9 and 2 are necessary for the migration of Langerhans cells and dermal dendritic cells from human and murine skin. Journal of Immunology 168(9): 4361-4371, 2002

Immunohistochemical study of epidermal Langerhans cells and dermal dendritic cells in benign and malignant skin lesions characterized by a dermal lymphoid infiltrate consisting either of B-cells or T-cells. Virchows Archiv. A Pathological Anatomy and Histopathology 411(4): 337-343, 1987

Chronic ethanol consumption decreases murine Langerhans cell numbers and delays migration of Langerhans cells as well as dermal dendritic cells. Alcoholism Clinical and Experimental Research 32(4): 657-668, 2008

The accumulation of Fc-epsilon-RI-alpha-bearing Langerhans and dermal dendritic cells is responsible for the overexpression of this receptor in atopic dermatitis skin. 9TH INTERNATIONAL CONGRESS OF IMMUNOLOGY [Author] The 9th International Congress of Immunology : 210, 1995

Langerhans cells and dermal dendritic cells capture protein antigens in the skin: possible targets for vaccination through the skin. Immunobiology 215(9-10): 770-779, 2011

Langerhans cells, dermal dendritic cells and monocyte-derived dendritic cells are distinct subsets of myeloid dendritic cells defined by diverse functional properties. Archives of Dermatological Research 295(8-9): 342, 2004

Relay of herpes simplex virus between Langerhans cells and dermal dendritic cells in human skin. Plos Pathogens 11(4): E1004812, 2015

Epidermal Langerhans' cells and dermal dendritic cells in human fetal and neonatal skin: an immunohistochemical study. Pediatric Dermatology 4(1): 11-17, 1987

Application of Microneedles to Skin Induces Activation of Epidermal Langerhans Cells and Dermal Dendritic Cells in Mice. Biological and Pharmaceutical Bulletin 39(8): 1309-1318, 2016

CD24a expression levels discriminate Langerhans cells from dermal dendritic cells in murine skin and lymph nodes. Journal of Investigative Dermatology 128(6): 1470-1475, 2008

Brugia malayi infective larvae fail to activate Langerhans cells and dermal dendritic cells in human skin. Parasite Immunology 37(2): 79-91, 2015

Effects of solar ultraviolet radiation on engineered human skin equivalent containing both Langerhans cells and dermal dendritic cells. Tissue Engineering 13(11): 2667-2679, 2007

Immunohistochemical and immunoelectron microscopic study of epidermal langerhans cells and dermal dendritic cells in murine fetal and neonatal skin. Thivolet, J And D Schmitt (Ed ) Colloque Inserm (Institut National De La Sante Et De La Recherche Medicale), Vol 172 La Cellule De Langerhans; (Inserm (National Institute Of Health And Medical Research) Colloquium), Vol 172 The Langerhans Cell; Second Workshop, Lyon, France, April 21-22, Xxv+492p Les Editions Inserm: Paris, France; John Libbey And Co Ltd : London, England, Uk Illus Paper 33-40, 1988

Epidermal Langerhans cells, dermal dendritic cells, and keratinocytes in viral lesions of skin and mucous membranes: an immunohistochemical study. Archives of Dermatological Research 280(4): 220-227, 1988