+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Modulation of double-stranded RNA-mediated gene induction by interferon in human umbilical vein endothelial cells



Modulation of double-stranded RNA-mediated gene induction by interferon in human umbilical vein endothelial cells



Journal of Interferon and Cytokine Research 20(11): 1007-1013



Endothelial cells respond to double-stranded RNA (dsRNA) with expression of a number of important immunomodulatory and inflammatory response genes, including adhesion molecules, cytokines, and antiviral genes. Considerable differences are seen when genes are induced by dsRNA compared with cytokines. Much higher levels of mRNA for interleukin-6 (IL-6), 2',5'-oligoadenylate synthetase (2',5'-OAS), protein kinase (PKR), and interferon (IFN) regulatory factor-1 (IRF-1) result from incubation with dsRNA than with IL-1beta, tumor necrosis factor-alpha (TNF-alpha), or IFN-alpha, whereas the differences in vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and E-selectin mRNA expression in response to dsRNA, IL-1beta, and TNF-alpha are relatively minor. IFN-alpha priming enhances responsiveness of some, but not all, genes to dsRNA but not to IL-1beta, but the optimal time for pretreatment varies considerably among different dsRNA-responsive genes. Protein translation is reduced in human umbilical vein endothelial cells (HUVEC) in response to incubation with dsRNA, and this decrease is accentuated if cells are primed with IFN-alpha. Despite this decrease, IFN-alpha priming causes very high levels of IL-6 protein expression in response to dsRNA but not in response to IL-1beta or TNF-alpha. These studies demonstrate that priming with class I IFN can enhance the response to dsRNA through the heightened expression of genes that contribute to both the cellular response to viral infection and the host immunologic response.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 010999111

Download citation: RISBibTeXText

PMID: 11096458

DOI: 10.1089/10799900050198453


Related references

Ifn- Induced Adenosine Production on the Endothelium: A Mechanism Mediated by Cd73 (Ecto-5'-Nucleotidase) Up-Regulation. The Journal of Immunology 172(3): 1646-1653, 2004

IFN-a Regulates TLR-Dependent Gene Expression of IFN-a, IFN-b, IL-28, and IL-29. Journal of Immunology 174(4): 32-7, 2005

IFN-alpha sensitizes Daudi B lymphoma cells to anti-IgM-induced loss of mitochondrial membrane potential through activation of c-Jun NH2-terminal kinase. Journal of Interferon and Cytokine Research 26(6): 421-429, 2006

Interferon does not affect rat small intestinal myoelectric activity. Gastroenterology 88(5 Part 2): 1373, 1985

Synthetic dna fragments and their use in molecular biology. Delaage, M Colloque Inserm (Institut National De La Sante Et De La Recherche Medicale), Vol 181 Interactions Chimie-Biologie: Bases Moleculaires Et Applications, 2 Mecanismes De Reconnaissance Moleculaire; (Inserm (National Institute Of Health And Medical Research) Colloquium, Vol 181 Interactions Between Biology And Chemistry: Molecular Bases And Applications: 2 Mechanisms Of Molecular Recognition); Summer School in Pharmacochemistry Vii+352p Les Editions Inserm: Paris, France I, 1989

Effects of Brazilian Cardioprotective Diet Program on risk factors in patients with coronary heart disease: a Brazilian Cardioprotective Diet randomized pilot trial. Clinics 67(12): 1407-1414, 2012

Tocopherol retention and vitamin E activity in frozen and canned immature seeds of five cultivars of common bean. 2012

A double suicide gene system driven by KDR promoter selectively kills human colon adneocarcinoma SW480 cells. Nan Fang Yi Ke Da Xue Xue Bao 30(2): 224-227, 2010

Induction of thioredoxin by ultraviolet-A radiation prevents oxidative-mediated cell death in human skin fibroblasts. Free Radical Biology and Medicine 31(5): 585-598, 2001

Liposome-mediated glial growth factor 2 gene therapy in brain injury: an experimental study with rats. Zhonghua Yi Xue Za Zhi 86(33): 2352-2356, 2006

Modulation of gene expression by alloimmune networks following murine heart transplantation. Molecular Genetics and Genomics 271(6): 687-696, 2004

Analysis of NF-ATc expression during thymic maturation and selection events. Journal of Cellular Biochemistry Suppl. 0(18D): 459, 1994

Induction of apoptosis by herpes simplex virus type 1. Journal of General Virology 78: 2909-2912, 1997

Effect of adenovirus-mediated up-regulation of α-enolase gene products on follicle-stimulating hormone receptor mRNA and luteinizing hormone receptor mRNA of granular cells from goose F1 follicles. Research in Veterinary Science 96(3): 526-532, 2014

Induction of interactions between CD44 and hyaluronic acid by a short exposure of human T cells to diverse pro-inflammatory mediators. Immunology 100(3): 345-351, 2000