Molecular cloning and construction of plant expression vector of chalcone synthase gene from Narcissus tazetta L. var. chinensis Roem
Huang Yin Yi; Shen Ming Shan; Chen Liang; Xiong Lin Yuan; Chen, M.Z.uan
Xiamen Daxue Xuebao (Ziran Kexue Ban) 41(5): 541-545
Chalcone synthase is a key enzyme in the biosynthesis of all classes of flavonoids. By RT-PCR, the cDNA sequence of CHS gene from Narcissus was cloned and the coding sequence of gene was further analyzed. The result demonstrated that the sequence of the coding region was 1 167 bp, encodes a protein consisting of 389 amino acid. In order to transfer CHS gene into plant, plant expression vector p1301BOMEGAC was constructed by one median clone.