Molecular cloning and sequencing of outer capside protein gene of rice dwarf virus and its expression in Escherichia coli

Lu-Ruifang; Li-Yi; Yang-Chonglin; Yan-Hua; Chen-Zhangliang

Weishengwu Xuebao 39(4): 305-314

1999


ISSN/ISBN: 0001-6209
Accession: 011005332

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Abstract
The S2 full-length cDNA of rice dwarf phytoreovirus which encodes the viral outer capsid protein was cloned and its complete nucleotide sequence was determined. The results showed that S2 is 3512bp long with a large open reading frame which encodes a proterin of 1116 amino acids. It shares 94.6% and 95.4% identity with RDV of Japanese H isolate in terms of nucleotide and amino acid sequences, respectively, and it also shows some homology with VP2 of rotavirus at the level of amino acid sequence. The search of deduced RDV S2 amino acid sequence in Blast network found that there were 4 leucine-rich motifs in P2 protein, and ten amino acids within the hydrophibic region at amino-terminus could form an alpha-helix. Predicted secondary structure of S2 cDNA indicated that a hairpin and a stem loop are present in the 5'-end within 50bp, and a stem loop in the 3'-end within 50bp. RDV S2 partial and full-length sequences were then cloned into expression vector pET-11d &pTrcHisC. SDS-PAGE and Western blot proved that amino- and carborn-termini of P2 were successfully expressed in E. coli.