+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Molecular recognition by the Candida albicans group I intron: Tertiary interactions with an imino GcntdotA pair facilitate binding of the 5' exon and lower the KM for guanosine



Molecular recognition by the Candida albicans group I intron: Tertiary interactions with an imino GcntdotA pair facilitate binding of the 5' exon and lower the KM for guanosine



Biochemistry 41(25): 8113-8119



A GcntdotA pair at position -5 in the P1 helix of the Candida albicans ribozyme contributes to tertiary binding of the 5' exon substrate (Disney, M. D., Haidaris, C. G., and Turner, D. H. (2001) Biochemistry 40, 6507-6519). Here, the G in the GcntdotA pair is replaced with inosine (I) in both semisynthetic ribozymes and oligonucleotide mimics of the internal guide sequence. Comparisons of oligonucleotide binding affinity for these and other sequences indicate that the GcntdotA pair is in an imino conformation where the exocyclic amine of G contributes apprx1.4 kcal/mol to tertiary interactions that help dock the ribozyme's P1 helix. Furthemore, replacement of the GcntdotA pair with a G-C pair produces less favorable interactions with the 2'-hydroxyl group at the -3 position and a less favorable KM for pG in a ribozyme-catalyzed transesterification reaction. These results are also consistent with the GcntdotA pair promoting docking of the P1 helix into the catalytic core. Evidently, tertiary interactions with the exocyclic amino group of a G in a single GcntdotA pair can increase the equilibrium constant for tertiary folding of RNA by roughly 10-fold at 37degreeC. Results with a GcntdotU or GcntdotG pair replacing the GcntdotA pair at the -5 position suggest similar tertiary interactions with these pairs.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 011010647

Download citation: RISBibTeXText


Related references

Molecular Recognition by the Candida albicans Group I Intron: Tertiary Interactions with an Imino GA Pair Facilitate Binding of the 5 Exon and Lower the K M for Guanosine. Biochemistry 41(25): 8113-8119, 2002

Molecular recognition by the Candida albicans group I intron: tertiary interactions with an imino G A pair facilitate binding of the 5' exon and lower the KM for guanosine. Biochemistry (American Chemical Society) 41(25): 13-19, 2002

Recognition elements for 5' exon substrate binding to the Candida albicans group I intron. Biochemistry Washington 40(21): 6507-6519, 2001

Binding enhancement by tertiary interactions and suicide inhibition of a Candida albicans group I intron by phosphoramidate and 2'-O-methyl hexanucleotides. Biochemistry 40(21): 6520-6526, 2001

Designing therapeutics based on molecular recognition of a Candida albicans group I intron. Abstracts of Papers American Chemical Society 220(Part 1): MEDI 103, 2000

From base-pair to tertiary interactions GcntdotA linkers in RNA structures. Biophysical Journal 86(1): 144a, 2004

Molecular characterization of new clinical isolates of Candida albicans and C. dubliniensis in Japan: analysis reveals a new genotype of C. albicans with group I intron. Journal of Clinical Microbiology 39(12): 4309-4315, 2001

A novel group I intron in Candida dubliniensis is homologous to a Candida albicans intron. Gene (amsterdam). 180(1-2): 189-196, 1996

NMR structure of the 5' splice site in the group IIB intron Sc.ai5γ--conformational requirements for exon-intron recognition. Rna 20(3): 295-307, 2014

Tight binding of the 5' exon to domain I of a group II self-splicing intron requires completion of the intron active site. Embo Journal 18(4): 1025-1037, 1999

Binding of a group II intron-encoded reverse transcriptase/maturase to its high affinity intron RNA binding site involves sequence-specific recognition and autoregulates translation. Journal of Molecular Biology 318(2): 287-303, 2002

Splicing of the mitochondrial group-II intron rI1: Conserved intron-exon interactions diminish splicing efficiency. Current Genetics 33(2): 117-123, 1998

Folding of the group I intron ribozyme from the 26S rRNA gene of Candida albicans. Nucleic Acids Research 29(12): 2644-2653, 2001

Group I intron renders differential susceptibility of Candida albicans to Bleomycin. Molecular Biology Reports 34(1): 11-17, 2007

In vivo recognition of a vertebrate mini-exon as an exon-intron-exon unit. Molecular and Cellular Biology 13(5): 2677-2687, 1993