Monolayers of derivatized poly (L-lysine) -grafted poly (ethylene glycol) on metal oxides as a class of biomolecular interfaces

Ruiz-Taylor, L.A.; Martin, T.L.; Zaugg, F.G.; Witte, K.; Indermuhle, P.; Nock, S.; Wagner, P.

Proceedings of the National Academy of Sciences of the United States of America 98(3): 852-857

2001


ISSN/ISBN: 0027-8424
PMID: 11158560
DOI: 10.1073/pnas.98.3.852
Accession: 011014106

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Abstract
We report on the design and characterization of a class of biomolecular interfaces based on derivatized poly(L-lysine)-grafted poly(ethylene glycol) copolymers adsorbed on negatively charged surfaces. As a model system, we synthesized biotin-derivatized poly(L-lysine)-grafted poly(ethylene glycol) copolymers, PLL-g-((PEGm)(1-x) (PEG-biotin)x), where x varies from 0 to 1. Monolayers were produced on titanium dioxide substrates and characterized by x-ray photoelectron spectroscopy. The specific biorecognition properties of these biotinylated surfaces were investigated with the use of radiolabeled streptavidin alone and within complex protein mixtures. The PLL-g-PEG-biotin monolayers specifically capture streptavidin, even from a complex protein mixture, while still preventing nonspecific adsorption of other proteins. This streptavidin layer can subsequently capture biotinylated proteins. Finally, with the use of microfluidic networks and protein arraying, we demonstrate the potential of this class of biomolecular interfaces for applications based on protein patterning.