EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Multi-dimensional liquid phase based separations in proteomics



Multi-dimensional liquid phase based separations in proteomics



Journal of Chromatography B 787(1): 11-18, 5 April



This review covers recent developments towards the implementation of multi-dimensional (MuD) liquid phase based systems for proteome investigations. Although two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) has been used as a standard approach in proteomics, its drawbacks including the limited dynamic range and molecular mass range, together with lack of on-line integration with biological mass spectrometery (Bio-MS) have limited its widespread use and applications in proteomics. In the meantime, various liquid-phase based multi-dimensional separation techniques have been explored. Especially, with the emergence of the combination of nanoflow capillary high-performance liquid chromatography (cHPLC) and Bio-MS, attention is again refocused on utilizing multi-dimensional liquid-phase based separation of proteins. Some remarkable applications of on-line analysis of intact proteins and on-column digested proteins, and the emergence of approaches such as multiple HPLC-electrospray ionization tandem MS and capillary array electrophoresis-matrix assisted laser desorption ionization MS, have stimulated thinking towards developing a automated multi-dimensional system (MuDSy) that integrates liquid phase based separation, digestion and identification of proteins in complex biological mixtures.

(PDF emailed within 0-6 h: $19.90)

Accession: 011022044

Download citation: RISBibTeXText

PMID: 12659729

DOI: 10.1016/s1570-0232(02)00335-5



Related references

Preparative two-dimensional liquid based proteomic separations using free-flow electrophoresis and reversed-phase high performance liquid chromatography. Molecular & Cellular Proteomics 2(9): 817, September, 2003

Differential proteomics incorporating iTRAQ labeling and multi-dimensional separations. Methods in Molecular Biology 691: 369-383, 2011

Multidimensional liquid phase separations of intact proteins as an alternative to 2D gel electrophoresis for proteomics. Molecular & Cellular Proteomics 2(7 Supplement): S37, July, 2003

Plasma proteomics of lung cancer by a linkage of multi-dimensional liquid chromatography and two-dimensional difference gel electrophoresis. Proteomics 6(13): 3938-3948, 2006

Evaluation of comprehensive multidimensional separations using reversed-phase, reversed-phase liquid chromatography/mass spectrometry for shotgun proteomics. Journal of Proteome Research 7(3): 1007-1011, 2008

Multidimensional liquid phase protein separations in conjunction with stable isotope labelling for quantitative proteomics. Proteomics 7(21): 3826-3834, 2007

Highly efficient peptide separations in proteomics. Part 2: bi- and multidimensional liquid-based separation techniques. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 877(11-12): 1019-1039, 2009

Multi-dimensional liquid chromatography in proteomics--a review. Analytica Chimica Acta 664(2): 101-113, 2010

Rapid identification and screening of proteins from whole cell lysates of human erythroleukemia cells in the liquid phase, using non-porous reversed phase high-performance liquid chromatography separations of proteins followed by multi-assisted laser desorption/ionization mass spectrometry analysis and sequence database searching. Rapid Communications in Mass Spectrometry 12(24): 1994-2003, 1998

A microfabricated fluidic device for performing two-dimensional liquid-phase separations. Analytical Chemistry 72(21): 5244-5249, 2000

Phase system selectivity and two-dimensional separations in liquid column chromatography. Journal of Chromatography. A 1087(1-2): 112-123, 2005

Fraction transfer process in on-line comprehensive two-dimensional liquid-phase separations. Journal of Separation Science 40(1): 109-123, 2016

Two dimensional liquid phase separations of proteins using online fractionation and concentration between chromatographic dimensions. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 847(2): 103-113, 2006

Construction and initial evaluation of an apparatus for spatial comprehensive two-dimensional liquid-phase separations. Analytica Chimica Acta 701(1): 92-97, 2011

Comprehensive two-dimensional separations of complex mixtures using reversed-phase reversed-phase liquid chromatography. Journal of Chromatography. A 1041(1-2): 101-110, 2004